MANFU12-6B5

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$40.00
SKU: MANFU12-6B5
View product citations for antibody MANFU12-6B5 on CiteAb

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Available: 178

DSHB Data Sheet

Catalog Fields

Antigen: Fukutin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a glycosyltransferase in the cis-Golgi compartment. Fukutin is thought to be responsible for the glycosylation of alpha-dystroglycan in skeletal muscle.
Antigen Molecular Weight: 53.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: FKTN
Immunogen: Recombinant Protein
Alternate Gene Names: FCMD, LGMD2M
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: a.a. 452-461 (SEWD – VI - - Y)
Uniprot ID: O75072 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 2218 
Antibody Registry ID: AB_2618193 
Additional Information: Epitope mapped by phage display. RRID:AB_2618193
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANFU12-6B5 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANFU12-6B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    IF References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    WB References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    Epitope Map References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    All References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

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