MANFU12-6B5

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SKU: MANFU12-6B5

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Available: 10

DSHB Data Sheet

Catalog Fields

Product Name/ID: MANFU12-6B5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: FKTN
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2618193 
Uniprot ID: O75072 
RRID:  
Entrez Gene ID: 2218 
Clonality: Monoclonal
Immunogen: Recombinant Protein
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Antigen Name: Fukutin
Epitope Location or Sequence: a.a. 452-461 (SEWD – VI - - Y)
Alternate Antigen Name:
Deposit Date: 3/27/2013
Antigen Molecular Weight: 53.7 kDa
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: Human
Depositor Notes: This antibody recognizes a glycosyltransferase in the cis-Golgi compartment. Fukutin is thought to be responsible for the glycosylation of alpha-dystroglycan in skeletal muscle.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Epitope mapped by phage display. RRID:AB_2618193
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANFU12-6B5 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANFU12-6B5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    IF References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    WB References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    Epitope Map References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    All References

    Detection of the dystroglycanopathy protein, fukutin, using a new panel of site-specific monoclonal antibodies.
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

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