MANFU8-2F5

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$40.00
SKU: MANFU8-2F5
View product citations for antibody MANFU8-2F5 on CiteAb

In Stock

Available: 180

DSHB Data Sheet

Catalog Fields

Antigen: Fukutin
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a glycosyltransferase in the cis-Golgi compartment. Fukutin is thought to be responsible for the glycosylation of alpha-dystroglycan in skeletal muscle.
Antigen Molecular Weight: 53.7 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: FKTN
Immunogen: Recombinant Protein
Alternate Gene Names: FCMD, LGMD2M
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: a.a. 180-187 (SGNYL - HG)
Uniprot ID: O75072 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 2218 
Additional Characterization: Link 
Antibody Registry ID: AB_2618199 
Additional Information: Epitope mapped by phage display. RRID:AB_2618199
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANFU8-2F5 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANFU8-2F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Biochemical and biophysical research communications
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    IF References

    Biochemical and biophysical research communications
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    WB References

    Biochemical and biophysical research communications
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    Epitope Map References

    Biochemical and biophysical research communications
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

    All References

    Biochemical and biophysical research communications
    Morris GE
    Biochemical and biophysical research communications 424.2 (2012 Jul 27): 354-7.

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