MANHINGE1B clone 10F9
Product Name/ID: MANHINGE1B clone 10F9
Available to For-Profits: Yes
Alternate Antibody Name:
Ab Isotype: MIgG1
Antibody Registry ID: AB_1157884
Immunogen: peptide AA255-270 LPRPPKVTKEEHFQLH conjugated to BSA
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Antigen Name: dystrophin
Epitope Location or Sequence: AA255-270 LPRPPKVTKEEHFQLH of human dystrophin
Alternate Antigen Name:
Deposit Date: 4/18/2007
Antigen Molecular Weight:
Depositor: Morris, G.E.
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: human
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Human
Additional Characterization: http://www.glennmorris.org.uk/datasheets/MANHINGE1B(10F9).doc
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANHINGE1B clone 10F9 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANHINGE1B clone 10F9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).