Antigen: Dystrophin (exon51)
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Depositor: Morris, G.E.
Host Species: mouse
Positive Tested Species Reactivity: Human
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Molecular Weight: 426.7 kDa
Depositors Notes: Internal fiber staining.
Immunogen: Recombinant dystrophin fragment
Alternate Antibody Name:
Alternate Gene Names: BMD
Alternate Antigen Name:
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: P11532
Epitope Location or Sequence: a.a. 2454-2468
Entrez Gene ID: 1756
Immunogen Sequence: Exon 51
Antibody Registry ID: AB_2753252
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANHINGE3C(10E12) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANHINGE3C(10E12))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).