MANNES1B(7B2)

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SKU: MANNES1B(7B2)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: MANNES1B(7B2)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: SYNE1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2753300 
Uniprot ID: Q8NF91 
RRID:  
Entrez Gene ID: 23345 
Clonality: Monoclonal
Immunogen: Nesprin-1alpha recombinant
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Nesprin-1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/9/2017
Antigen Molecular Weight: 1,011.1 kDa
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: Human
Depositor Notes: This antigen is also known as enaptin or myocyte nuclear encelope protein 1.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANNES1B(7B2) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANNES1B(7B2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

    IF References

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

    All References

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

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