MANNES2G(4B5)

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$40.00
SKU: MANNES2G(4B5)
View product citations for antibody MANNES2G(4B5) on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Nesprin-2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Host Species: mouse
Depositors Notes: This antigen is also known as nuclear envelope spectrin repeat protein 2 or NUANCE.
Positive Tested Species Reactivity: Human
Gene: SYNE2
Antigen Molecular Weight: 796.4 Kda
Alternate Gene Names: NUA
Immunogen: Recombinant protein fragment
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: Q8WXH0 
Epitope Location or Sequence: a.a . 6694-6701
Entrez Gene ID: 23224 
Immunogen Sequence: Nt 19,831-20,853; C-terminal 38 kDa fragment
Recommended Applications: Immunofluorescence, Western Blot
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANNES2G(4B5) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANNES2G(4B5))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

    IF References

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

    WB References

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

    Epitope Map References

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

    All References

    Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development.
    Morris GE
    Developmental dynamics : an official publication of the American Association of Anatomists 239.3 (2010 Mar): 998-1009.

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