MANSIP1A(1G9)

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$40.00
SKU: MANSIP1A(1G9)
View product citations for antibody MANSIP1A(1G9) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Gem-associated protein 2 (Gemin2)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Host Species: mouse
Depositors Notes: Gemin2 (Gem nuclear organelle associated protein 2) is part of the SMN complex in motor neurons. Mutations in this complex lead to childhood spinal muscular atrophy.
Positive Tested Species Reactivity: Human, Mouse, Rabbit
Gene: GEMIN2
Antigen Molecular Weight: 31.6kDa
Alternate Gene Names: SIP1
Immunogen: Recombinant protein
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: No
Uniprot ID: O14893 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 8487 
Antibody Registry ID: AB_2618222 
Additional Information: RRID:AB_2618222
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANSIP1A(1G9) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANSIP1A(1G9))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

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