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View product citations for antibody MANSMA1(11F3) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: SMN1 protein, exon 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK
Host Species: mouse
Depositors Notes: Stains SMN band in HeLa total protein extract (with 2 higher Mr bands). Stains dot-like structures (gems) in the nucleus. The staining of mouse SMN is weak. Doesn'r work on rabbit.
Positive Tested Species Reactivity: Human, Mouse, Porcine, Rat
Gene: SMN1
Antigen Molecular Weight: Predicted: 32 kDa; Apparent: 38 kDa
Alternate Gene Names: SMN, SMNT, SMN2, SMNC, Gemin1
Immunogen: Recombinant SMN protein
Clonality: Monoclonal
Alternate Antibody Name: MANSMA1
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: exon 2, aa 28-91 (conformational epitope)
Uniprot ID: Q16637 
Immunogen Sequence: Full length protein
Entrez Gene ID: 6606 6607 
Antibody Registry ID: AB_2618227 
Additional Information: Contrary to what was published in the initial publication, the Depositor stated that this antibody doesn't work on rabbit (see Depositor notes).
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANSMA1(11F3) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANSMA1(11F3))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    The relationship between SMN, the spinal muscular atrophy protein, and nuclear coiled bodies in differentiated tissues and cultured cells.
    Morris GE
    Experimental cell research 256.2 (2000 May 1): 365-74.

    Dilysine motifs in exon 2b of SMN protein mediate binding to the COPI vesicle protein α-COP and neurite outgrowth in a cell culture model of spinal muscular atrophy.
    Androphy EJ
    Human molecular genetics 22.20 (2013 Oct 15): 4043-52.

    Survival motor neuron protein in the nucleolus of mammalian neurons.
    Francis JW
    Brain research 945.2 (2002 Aug 2): 160-73.

    Altered mRNA Splicing in SMN-Depleted Motor Neuron-Like Cells.
    Androphy EJ
    PloS one 11.10 (2016): e0163954.

    Drosophila SMN2 minigene reporter model identifies moxifloxacin as a candidate therapy for SMA.
    Artero R
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology . (2019 Dec 31): .

    IP References
    ELISA References
    Epitope Map References
    All References

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