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DSHB Data Sheet

Catalog Fields

Product Name/ID: MANSMA1(11F3)
Available to For-Profits: Yes
Alternate Antibody Name: MANSMA1
Gene Name: SMN1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2618227 
Uniprot ID: Q16637 
Entrez Gene ID: 6606 6607 
Clonality: Monoclonal
Immunogen: Recombinant SMN protein
Immunogen Sequence: Full length protein
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Antigen Name: SMN1 protein, exon 2
Epitope Location or Sequence: exon 2, aa 28-91 (conformational epitope)
Alternate Antigen Name:
Deposit Date: 6/13/2011
Antigen Molecular Weight: Predicted: 32 kDa; Apparent: 38 kDa
Depositor: Morris, G.E.
Antigen Sequence:
Depositor Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Species: Human
Depositor Notes: Stains SMN band in HeLa total protein extract (with 2 higher Mr bands). Stains dot-like structures (gems) in the nucleus. The staining of mouse SMN is weak. Doesn'r work on rabbit.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Porcine, Rat
Additional Information: Contrary to what was published in the initial publication, the Depositor stated that this antibody doesn't work on rabbit (see Depositor notes).
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANSMA1(11F3) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANSMA1(11F3))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    The relationship between SMN, the spinal muscular atrophy protein, and nuclear coiled bodies in differentiated tissues and cultured cells.
    Morris GE
    Experimental cell research 256.2 (2000 May 1): 365-74.

    Dilysine motifs in exon 2b of SMN protein mediate binding to the COPI vesicle protein α-COP and neurite outgrowth in a cell culture model of spinal muscular atrophy.
    Androphy EJ
    Human molecular genetics 22.20 (2013 Oct 15): 4043-52.

    Survival motor neuron protein in the nucleolus of mammalian neurons.
    Francis JW
    Brain research 945.2 (2002 Aug 2): 160-73.

    Altered mRNA Splicing in SMN-Depleted Motor Neuron-Like Cells.
    Androphy EJ
    PloS one 11.10 (2016): e0163954.

    Drosophila SMN2 minigene reporter model identifies moxifloxacin as a candidate therapy for SMA.
    Artero R
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34.2 (2020 Feb): 3021-3036.

    IP References
    ELISA References
    Epitope Map References
    All References

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