MANSMA13(5E3)

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$40.00
SKU: MANSMA13(5E3)
View product citations for antibody MANSMA13(5E3) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: SMN1 protein, exon 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: Stains only SMN band in HeLa total protein extract. Stains dot-like structures (gems) in the nucleus.
Antigen Molecular Weight: Predicted: 32 kDa; Apparent: 38 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SMN1
Immunogen: Recombinant SMN protein
Alternate Gene Names: SMN, SMNT, SMN2, SMNC, Gemin1
Alternate Antibody Name: MANSMA13
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: exon 2, aa 28-91 (conformational epitope)
Uniprot ID: Q16637 
Immunogen Sequence: Full length protein
Entrez Gene ID: 6606 6607 
Antibody Registry ID: AB_2618231 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANSMA13(5E3) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANSMA13(5E3))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
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