MANSMA16(8A6)

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$40.00
SKU: MANSMA16(8A6)
View product citations for antibody MANSMA16(8A6) on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: SMN1 protein, exon 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG1
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, UK
Host Species: mouse
Depositors Notes: Stains only SMN band in HeLa total protein extract. Stains dot-like structures (gems) in the nucleus.
Positive Tested Species Reactivity: Human, Mouse
Gene: SMN1
Antigen Molecular Weight: Predicted: 32 kDa; Apparent: 38 kDa
Alternate Gene Names: SMN, SMNT, SMN2, SMNC, Gemin1
Immunogen: Recombinant SMN protein
Clonality: Monoclonal
Alternate Antibody Name: MANSMA16
Epitope Mapped: Yes
Myeloma Strain: Sp2/0
Epitope Location or Sequence: exon 2, aa 28-91 (conformational epitope)
Uniprot ID: Q16637 
Immunogen Sequence: Full length protein
Entrez Gene ID: 6606 6607 
Antibody Registry ID: AB_2618234 
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MANSMA16(8A6) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MANSMA16(8A6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    ELISA References
    Epitope Map References
    All References

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