MAb1403

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$40.00
SKU: MAb1403
View product citations for antibody MAb1403 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Vesicular acetylcholine transporter unc-17
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Rand, J.B. / Miller, K. G.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Oklahoma Center for Neuroscience (OHUSC)
Positive Tested Species Reactivity: C. elegans
Depositors Notes: This antigen is also known as uncoordinated protein 17. Supernatant (37 ug/ml) may be used at 1:500 dilution for immunostaining.
Antigen Molecular Weight: 58.6 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: unc-17
Immunogen: E. coli-expressed MBP-fusion protein
Alternate Gene Names: ZC416.8
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: NPHRRGTDSHGEKVQGT
Uniprot ID: P34711 
Immunogen Sequence: NPHRRGTDSHGEKVQGT
Entrez Gene ID: 24105312 
Additional Characterization:  
Antibody Registry ID: AB_2315531 
Additional Information: RRID:AB_2315531

This antibody is also useful for immunoelectron microscopy (see PMID: 14688212).
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MAb1403 was deposited to the DSHB by Rand, J.B. / Miller, K. G. (DSHB Hybridoma Product MAb1403)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

26 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Identification of major classes of cholinergic neurons in the nematode Caenorhabditis elegans.
    Rand JB
    The Journal of comparative neurology 506.3 (2008 Jan 20): 398-408.

    IF References

    Identified neurons in C. elegans coexpress vesicular transporters for acetylcholine and monoamines.
    Rand JB
    American journal of physiology. Cell physiology 280.6 (2001 Jun): C1616-22.

    Presynaptic UNC-31 (CAPS) is required to activate the G alpha(s) pathway of the Caenorhabditis elegans synaptic signaling network.
    Miller KG
    Genetics 172.2 (2006 Feb): 943-61.

    The Dunce cAMP phosphodiesterase PDE-4 negatively regulates G alpha(s)-dependent and G alpha(s)-independent cAMP pools in the Caenorhabditis elegans synaptic signaling network.
    Miller KG
    Genetics 173.1 (2006 May): 111-30.

    Impaired dense core vesicle maturation in Caenorhabditis elegans mutants lacking Rab2.
    Miller KG
    The Journal of cell biology 186.6 (2009 Sep 21): 881-95.

    An organelle gatekeeper function for Caenorhabditis elegans UNC-16 (JIP3) at the axon initial segment.
    Miller KG
    Genetics 194.1 (2013 May): 143-61.

    A novel CaM kinase II pathway controls the location of neuropeptide release from Caenorhabditis elegans motor neurons.
    Miller KG
    Genetics 196.3 (2014 Mar): 745-65.

    Preventing Illegitimate Extrasynaptic Acetylcholine Receptor Clustering Requires the RSU-1 Protein.
    Bessereau JL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 36.24 (2016 Jun 15): 6525-37.

    C. elegans Punctin Clusters GABA(A) Receptors via Neuroligin Binding and UNC-40/DCC Recruitment.
    Bessereau JL
    Neuron 86.6 (2015 Jun 17): 1407-19.

    C. elegans Punctin specifies cholinergic versus GABAergic identity of postsynaptic domains.
    Bessereau JL
    Nature 511.7510 (2014 Jul 24): 466-70.

    Identification of major classes of cholinergic neurons in the nematode Caenorhabditis elegans.
    Rand JB
    The Journal of comparative neurology 506.3 (2008 Jan 20): 398-408.

    The basement membrane components nidogen and type XVIII collagen regulate organization of neuromuscular junctions in Caenorhabditis elegans.
    Kramer JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.9 (2003 May 1): 3577-87.

    An ER-resident membrane protein complex regulates nicotinic acetylcholine receptor subunit composition at the synapse.
    Gottschalk A
    The EMBO journal 28.17 (2009 Sep 2): 2636-49.

    Presynaptic UNC-31 (CAPS) is required to activate the G alpha(s) pathway of the Caenorhabditis elegans synaptic signaling network.
    Miller KG
    Genetics 172.2 (2006 Feb): 943-61.

    The Dunce cAMP phosphodiesterase PDE-4 negatively regulates G alpha(s)-dependent and G alpha(s)-independent cAMP pools in the Caenorhabditis elegans synaptic signaling network.
    Miller KG
    Genetics 173.1 (2006 May): 111-30.

    LAD-1, the Caenorhabditis elegans L1CAM homologue, participates in embryonic and gonadal morphogenesis and is a substrate for fibroblast growth factor receptor pathway-dependent phosphotyrosine-based signaling.
    Bennett V
    The Journal of cell biology 154.4 (2001 Aug 20): 841-55.

    Identified neurons in C. elegans coexpress vesicular transporters for acetylcholine and monoamines.
    Rand JB
    American journal of physiology. Cell physiology 280.6 (2001 Jun): C1616-22.

    Impaired dense core vesicle maturation in Caenorhabditis elegans mutants lacking Rab2.
    Miller KG
    The Journal of cell biology 186.6 (2009 Sep 21): 881-95.

    An organelle gatekeeper function for Caenorhabditis elegans UNC-16 (JIP3) at the axon initial segment.
    Miller KG
    Genetics 194.1 (2013 May): 143-61.

    Regulation of nicotinic receptor trafficking by the transmembrane Golgi protein UNC-50.
    Bessereau JL
    The EMBO journal 26.20 (2007 Oct 17): 4313-23.

    A transmembrane protein required for acetylcholine receptor clustering in Caenorhabditis elegans.
    Bessereau JL
    Nature 431.7008 (2004 Sep 30): 578-82.

    Tomosyn inhibits synaptic vesicle priming in Caenorhabditis elegans.
    Richmond JE
    PLoS biology 4.8 (2006 Jul): e261.

    Neuroligin-deficient mutants of C. elegans have sensory processing deficits and are hypersensitive to oxidative stress and mercury toxicity.
    Rand JB
    Disease models & mechanisms 3.5-6 (2010 May-Jun): 366-76.

    Expression of multiple UNC-13 proteins in the Caenorhabditis elegans nervous system.
    Rand JB
    Molecular biology of the cell 11.10 (2000 Oct): 3441-52.

    Regulation of neurotransmitter vesicles by the homeodomain protein UNC-4 and its transcriptional corepressor UNC-37/groucho in Caenorhabditis elegans cholinergic motor neurons.
    Miller DM 3rd
    The Journal of neuroscience : the official journal of the Society for Neuroscience 21.6 (2001 Mar 15): 2001-14.

    A conserved postsynaptic transmembrane protein affecting neuromuscular signaling in Caenorhabditis elegans.
    Hobert O
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.9 (2004 Mar 3): 2191-201.

    Differential expression and function of synaptotagmin 1 isoforms in Caenorhabditis elegans.
    Rand JB
    Molecular and cellular neurosciences 34.4 (2007 Apr): 642-52.

    The Caenorhabditis elegans snf-11 gene encodes a sodium-dependent GABA transporter required for clearance of synaptic GABA.
    Rand JB
    Molecular biology of the cell 17.7 (2006 Jul): 3021-30.

    Choline transport and de novo choline synthesis support acetylcholine biosynthesis in Caenorhabditis elegans cholinergic neurons.
    Rand JB
    Genetics 177.1 (2007 Sep): 195-204.

    UNC-41/stonin functions with AP2 to recycle synaptic vesicles in Caenorhabditis elegans.
    Rand JB
    PloS one 7.7 (2012): e40095.

    Preventing Illegitimate Extrasynaptic Acetylcholine Receptor Clustering Requires the RSU-1 Protein.
    Bessereau JL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 36.24 (2016 Jun 15): 6525-37.

    C. elegans Punctin specifies cholinergic versus GABAergic identity of postsynaptic domains.
    Bessereau JL
    Nature 511.7510 (2014 Jul 24): 466-70.

    A single immunoglobulin-domain protein required for clustering acetylcholine receptors in C. elegans.
    Bessereau JL
    The EMBO journal 30.4 (2011 Feb 16): 706-18.

    C. elegans Punctin Clusters GABA(A) Receptors via Neuroligin Binding and UNC-40/DCC Recruitment.
    Bessereau JL
    Neuron 86.6 (2015 Jun 17): 1407-19.

    WB References

    Identification of major classes of cholinergic neurons in the nematode Caenorhabditis elegans.
    Rand JB
    The Journal of comparative neurology 506.3 (2008 Jan 20): 398-408.

    IHC References
    Epitope Map References

    Identification of major classes of cholinergic neurons in the nematode Caenorhabditis elegans.
    Rand JB
    The Journal of comparative neurology 506.3 (2008 Jan 20): 398-408.

    All References

    The basement membrane components nidogen and type XVIII collagen regulate organization of neuromuscular junctions in Caenorhabditis elegans.
    Kramer JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 23.9 (2003 May 1): 3577-87.

    A regulatory cascade of three homeobox genes, ceh-10, ttx-3 and ceh-23, controls cell fate specification of a defined interneuron class in C. elegans.
    Hobert O
    Development (Cambridge, England) 128.11 (2001 Jun): 1951-69.

    Identified neurons in C. elegans coexpress vesicular transporters for acetylcholine and monoamines.
    Rand JB
    American journal of physiology. Cell physiology 280.6 (2001 Jun): C1616-22.

    Presynaptic UNC-31 (CAPS) is required to activate the G alpha(s) pathway of the Caenorhabditis elegans synaptic signaling network.
    Miller KG
    Genetics 172.2 (2006 Feb): 943-61.

    The Dunce cAMP phosphodiesterase PDE-4 negatively regulates G alpha(s)-dependent and G alpha(s)-independent cAMP pools in the Caenorhabditis elegans synaptic signaling network.
    Miller KG
    Genetics 173.1 (2006 May): 111-30.

    Impaired dense core vesicle maturation in Caenorhabditis elegans mutants lacking Rab2.
    Miller KG
    The Journal of cell biology 186.6 (2009 Sep 21): 881-95.

    An organelle gatekeeper function for Caenorhabditis elegans UNC-16 (JIP3) at the axon initial segment.
    Miller KG
    Genetics 194.1 (2013 May): 143-61.

    A novel CaM kinase II pathway controls the location of neuropeptide release from Caenorhabditis elegans motor neurons.
    Miller KG
    Genetics 196.3 (2014 Mar): 745-65.

    Preventing Illegitimate Extrasynaptic Acetylcholine Receptor Clustering Requires the RSU-1 Protein.
    Bessereau JL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 36.24 (2016 Jun 15): 6525-37.

    C. elegans Punctin Clusters GABA(A) Receptors via Neuroligin Binding and UNC-40/DCC Recruitment.
    Bessereau JL
    Neuron 86.6 (2015 Jun 17): 1407-19.

    C. elegans Punctin specifies cholinergic versus GABAergic identity of postsynaptic domains.
    Bessereau JL
    Nature 511.7510 (2014 Jul 24): 466-70.

    Identification of major classes of cholinergic neurons in the nematode Caenorhabditis elegans.
    Rand JB
    The Journal of comparative neurology 506.3 (2008 Jan 20): 398-408.

    An ER-resident membrane protein complex regulates nicotinic acetylcholine receptor subunit composition at the synapse.
    Gottschalk A
    The EMBO journal 28.17 (2009 Sep 2): 2636-49.

    LAD-1, the Caenorhabditis elegans L1CAM homologue, participates in embryonic and gonadal morphogenesis and is a substrate for fibroblast growth factor receptor pathway-dependent phosphotyrosine-based signaling.
    Bennett V
    The Journal of cell biology 154.4 (2001 Aug 20): 841-55.

    Regulation of nicotinic receptor trafficking by the transmembrane Golgi protein UNC-50.
    Bessereau JL
    The EMBO journal 26.20 (2007 Oct 17): 4313-23.

    A transmembrane protein required for acetylcholine receptor clustering in Caenorhabditis elegans.
    Bessereau JL
    Nature 431.7008 (2004 Sep 30): 578-82.

    Tomosyn inhibits synaptic vesicle priming in Caenorhabditis elegans.
    Richmond JE
    PLoS biology 4.8 (2006 Jul): e261.

    Neuroligin-deficient mutants of C. elegans have sensory processing deficits and are hypersensitive to oxidative stress and mercury toxicity.
    Rand JB
    Disease models & mechanisms 3.5-6 (2010 May-Jun): 366-76.

    Expression of multiple UNC-13 proteins in the Caenorhabditis elegans nervous system.
    Rand JB
    Molecular biology of the cell 11.10 (2000 Oct): 3441-52.

    Regulation of neurotransmitter vesicles by the homeodomain protein UNC-4 and its transcriptional corepressor UNC-37/groucho in Caenorhabditis elegans cholinergic motor neurons.
    Miller DM 3rd
    The Journal of neuroscience : the official journal of the Society for Neuroscience 21.6 (2001 Mar 15): 2001-14.

    A conserved postsynaptic transmembrane protein affecting neuromuscular signaling in Caenorhabditis elegans.
    Hobert O
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.9 (2004 Mar 3): 2191-201.

    Differential expression and function of synaptotagmin 1 isoforms in Caenorhabditis elegans.
    Rand JB
    Molecular and cellular neurosciences 34.4 (2007 Apr): 642-52.

    The Caenorhabditis elegans snf-11 gene encodes a sodium-dependent GABA transporter required for clearance of synaptic GABA.
    Rand JB
    Molecular biology of the cell 17.7 (2006 Jul): 3021-30.

    Choline transport and de novo choline synthesis support acetylcholine biosynthesis in Caenorhabditis elegans cholinergic neurons.
    Rand JB
    Genetics 177.1 (2007 Sep): 195-204.

    UNC-41/stonin functions with AP2 to recycle synaptic vesicles in Caenorhabditis elegans.
    Rand JB
    PloS one 7.7 (2012): e40095.

    A single immunoglobulin-domain protein required for clustering acetylcholine receptors in C. elegans.
    Bessereau JL
    The EMBO journal 30.4 (2011 Feb 16): 706-18.

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