MAb1403
Catalog Fields
Product Name/ID: MAb1403
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: unc-17
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2315531
Uniprot ID: P34711
Entrez Gene ID: 24105312
Clonality: Monoclonal
Immunogen: E. coli-expressed MBP-fusion protein
Clone:
Immunogen Sequence: NPHRRGTDSHGEKVQGT
Myeloma Strain:
Epitope Mapped: Yes
Antigen Name: Vesicular acetylcholine transporter unc-17
Epitope Location or Sequence: NPHRRGTDSHGEKVQGT
Alternate Antigen Name:
Deposit Date: 4/10/2018
Antigen Molecular Weight: 58.6 kDa
Depositor: Rand, J.B. / Miller, K. G.
Antigen Sequence:
Depositor Institution: Oklahoma Health Sciences Center / Oklahoma Center for Neuroscience
Antigen Species: C. elegans
Depositor Notes: This antigen is also known as uncoordinated protein 17. Supernatant (37 ug/ml) may be used at 1:500 dilution for immunostaining.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans
Additional Information: RRID:AB_2315531
This antibody is also useful for immunoelectron microscopy (see PMID: 14688212).
This antibody is also useful for immunoelectron microscopy (see PMID: 14688212).
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MAb1403 was deposited to the DSHB by Rand, J.B. / Miller, K. G. (DSHB Hybridoma Product MAb1403)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
26 References
Initial Publication
Identification of major classes of cholinergic neurons in the nematode Caenorhabditis elegans.
Rand JB
The Journal of comparative neurology 506.3 (2008 Jan 20): 398-408.