MAb1403
Catalog Fields
Antigen: Vesicular acetylcholine transporter unc-17
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Rand, J.B. / Miller, K. G.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Oklahoma Center for Neuroscience (OHUSC)
Positive Tested Species Reactivity: C. elegans
Depositors Notes: This antigen is also known as uncoordinated protein 17. Supernatant (37 ug/ml) may be used at 1:500 dilution for immunostaining.
Predicted Species Reactivity:
Gene: unc-17
Immunogen: E. coli-expressed MBP-fusion protein
Alternate Gene Names: ZC416.8
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: NPHRRGTDSHGEKVQGT
Uniprot ID: P34711
Immunogen Sequence: NPHRRGTDSHGEKVQGT
Entrez Gene ID: 24105312
Antibody Registry ID: AB_2315531
Additional Information: RRID:AB_2315531
This antibody is also useful for immunoelectron microscopy (see PMID: 14688212).
This antibody is also useful for immunoelectron microscopy (see PMID: 14688212).
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MAb1403 was deposited to the DSHB by Rand, J.B. / Miller, K. G. (DSHB Hybridoma Product MAb1403)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
26 References
Initial Publication
Identification of major classes of cholinergic neurons in the nematode Caenorhabditis elegans.
Rand JB
The Journal of comparative neurology 506.3 (2008 Jan 20): 398-408.