MB2b(6A10)

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$45.00 to $425.00
SKU: MB2b(6A10)
View product citations for antibody MB2b(6A10) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Muscleblind-like protein 2
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b
Depositor: Morris, G.E.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital
Antigen Molecular Weight: 40.5 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant protein
Gene: MBNL2
Alternate Antibody Name:
Alternate Gene Names: MBLL, MBLL39
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: Q5VZF2 
Epitope Location or Sequence: a.a. 132-135 by phage display
Entrez Gene ID: 10150 
Immunogen Sequence: MBNL2 exons 1, 2,3 and part of exon 4.
Antibody Registry ID: AB_2753297 
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Recommended Applications: Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MB2b(6A10) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MB2b(6A10))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle.
    Morris GE
    The American journal of pathology 174.1 (2009 Jan): 216-27.

    WB References

    Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle.
    Morris GE
    The American journal of pathology 174.1 (2009 Jan): 216-27.

    Epitope Map References

    Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle.
    Morris GE
    The American journal of pathology 174.1 (2009 Jan): 216-27.

    All References

    Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle.
    Morris GE
    The American journal of pathology 174.1 (2009 Jan): 216-27.

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