MB2b(6A10)

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$40.00
SKU: MB2b(6A10)
View product citations for antibody MB2b(6A10) on CiteAb

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Available: 6

DSHB Data Sheet

Catalog Fields

Antigen: Muscleblind-like protein 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Morris, G.E.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry, SY10 7AG, UK
Positive Tested Species Reactivity: Human
Depositors Notes:
Antigen Molecular Weight: 40.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MBNL2
Immunogen: Recombinant protein
Alternate Gene Names: MBLL, MBLL39
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 132-135 by phage display
Uniprot ID: Q5VZF2 
Immunogen Sequence: MBNL2 exons 1, 2,3 and part of exon 4.
Entrez Gene ID: 10150 
Additional Characterization: http://www.glennmorris.org.uk/mabs/WCIND.htm 
Antibody Registry ID: AB_2753297 
Additional Information:
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MB2b(6A10) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product MB2b(6A10))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle.
    Morris GE
    The American journal of pathology 174.1 (2009 Jan): 216-27.

    WB References

    Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle.
    Morris GE
    The American journal of pathology 174.1 (2009 Jan): 216-27.

    Epitope Map References

    Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle.
    Morris GE
    The American journal of pathology 174.1 (2009 Jan): 216-27.

    All References

    Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle.
    Morris GE
    The American journal of pathology 174.1 (2009 Jan): 216-27.

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