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View product citations for antibody MH4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: intermediate filament subunit
Hybridoma Cells Available: Yes
Antigen Species: Caenorhabditis elegans
Depositor: Waterston, R.H.
Isotype: MIgG
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Washington
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Cell binding note: hypodermis, marginal cells of the pharynx, vulva, uterus, a few cells in the tail.
Antigen Molecular Weight: 68-70 kDa by immunoblot
Human Protein Atlas:  
Predicted Species Reactivity:  
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/O-ag
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528312 
Additional Information:
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MH4 was deposited to the DSHB by Waterston, R.H. (DSHB Hybridoma Product MH4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Muscle cell attachment in Caenorhabditis elegans.
    Waterston RH
    The Journal of cell biology 114.3 (1991 Aug): 465-79.

    All References

    Muscle cell attachment in Caenorhabditis elegans.
    Waterston RH
    The Journal of cell biology 114.3 (1991 Aug): 465-79.

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