Catalog Fields
Antigen: Integrin alpha-L; CD11a
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Hildreth, J.E.K.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes: CD11a, as as component of the LFA-1 integrin, is the receptor for ISG15 protein (PMID: 29100055) to signal cytokine secretion. This antibody does recognizes CD11a in acetone-fixed frozen sections but fails in all aldehyde-fixation protocols.
Predicted Species Reactivity:
Gene: CD11A
Immunogen: EBV-transformed human lymphoblastoid cells
Alternate Gene Names: LFA-1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-NSI/1Ag4.1
Epitope Location or Sequence: alpha chain I domain; a.a.197-201
Uniprot ID: P20701
Immunogen Sequence: Full length sequence
Entrez Gene ID: 3683
Antibody Registry ID: AB_2265116
Additional Information: RRID: AB_2265116
Recommended Applications: FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MHM.24 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product MHM.24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
8 References
Initial Publication
A human lymphocyte-associated antigen involved in cell-mediated lympholysis.
McMichael AJ
European journal of immunology 13.3 (1983 Mar): 202-8.