MHM.24

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$40.00
SKU: MHM.24-s
View product citations for antibody MHM.24 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Integrin alpha-L; CD11a
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Hildreth, J.E.K.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes: CD11a, as as component of the LFA-1 integrin, is the receptor for ISG15 protein (PMID: 29100055) to signal cytokine secretion. This antibody does recognizes CD11a in acetone-fixed frozen sections but fails in all aldehyde-fixation protocols.
Antigen Molecular Weight: 175 kD
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CD11A
Immunogen: EBV-transformed human lymphoblastoid cells
Alternate Gene Names: LFA-1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3-NSI/1Ag4.1
Epitope Location or Sequence: alpha chain I domain; a.a.197-201
Uniprot ID: P20701 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 3683 
Additional Characterization:  
Antibody Registry ID: AB_2265116 
Additional Information: RRID: AB_2265116
Recommended Applications: FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MHM.24 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product MHM.24)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    A human lymphocyte-associated antigen involved in cell-mediated lympholysis.
    McMichael AJ
    European journal of immunology 13.3 (1983 Mar): 202-8.

    IF References
    WB References

    Extracellular ISG15 Signals Cytokine Secretion through the LFA-1 Integrin Receptor.
    Huibregtse JM
    Molecular cell 68.3 (2017 Nov 2): 581-590.e5.

    A human lymphocyte-associated antigen involved in cell-mediated lympholysis.
    McMichael AJ
    European journal of immunology 13.3 (1983 Mar): 202-8.

    IHC References

    A human lymphocyte-associated antigen involved in cell-mediated lympholysis.
    McMichael AJ
    European journal of immunology 13.3 (1983 Mar): 202-8.

    IP References
    FB References
    FACS References
    Epitope Map References
    All References

    A human lymphocyte-associated antigen involved in cell-mediated lympholysis.
    McMichael AJ
    European journal of immunology 13.3 (1983 Mar): 202-8.

    Identification and characterization of a human monoclonal antagonistic antibody AL-57 that preferentially binds the high-affinity form of lymphocyte function-associated antigen-1.
    Cohen EH
    Journal of leukocyte biology 80.4 (2006 Oct): 905-14.

    Human T-Cell Leukemia Virus Type 1 (HTLV-1) bZIP Factor Upregulates the Expression of ICAM-1 To Facilitate HTLV-1 Infection.
    Polakowski N
    Journal of virology 93.19 (2019 Oct 1): .

    Extracellular ISG15 Signals Cytokine Secretion through the LFA-1 Integrin Receptor.
    Huibregtse JM
    Molecular cell 68.3 (2017 Nov 2): 581-590.e5.

    Monoclonal antibodies against porcine LFA-1: species cross-reactivity and functional effects of beta-subunit-specific antibodies.
    Pescovitz MD
    Molecular immunology 26.9 (1989 Sep): 883-95.

    The human lymphocyte function-associated (HLFA) antigen and a related macrophage differentiation antigen (HMac-1): functional effects of subunit-specific monoclonal antibodies.
    August JT
    Journal of immunology (Baltimore, Md. : 1950) 134.5 (1985 May): 3272-80.

    Effects of monoclonal antibodies to the alpha and beta chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions.
    McMichael AJ
    European journal of immunology 15.9 (1985 Sep): 888-92.

    LFA-1 integrin antibodies inhibit leukocyte α4β1-mediated adhesion by intracellular signaling.
    Gahmberg CG
    Blood 128.9 (2016 Sep 1): 1270-81.

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