MRN3 3F10

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SKU: MRN3 3F10
View product citations for antibody MRN3 3F10 on CiteAb

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Available: 41

DSHB Data Sheet

Catalog Fields

Antigen: mineralocorticoid receptor
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Gomez-Sanchez, C.
Isotype: MIgG1, kappa light chain
Depositors Institution: G.V. Montgomery VA Medical Center
Host Species: mouse
Depositors Notes: Doesn't work for western blotting.
Positive Tested Species Reactivity: Mouse, Rat
Gene: Nr3c2
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Alternate Gene Names: Mlr, MCR
Clonality: Monoclonal
Alternate Antibody Name: rMR 79–97 3F10
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence: N-terminus A/B domain (aa 79-97)
Uniprot ID: P22199 
Immunogen Sequence: Partial protein
Entrez Gene ID: 25672 
Additional Information: Should recognize all MR isoforms. The sequence of the rMR 79–97 peptide is identical between rat and mouse, but differs in human.
Antibody Registry ID: AB_1157908 
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MRN3 3F10 was deposited to the DSHB by Gomez-Sanchez, C. (DSHB Hybridoma Product MRN3 3F10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IHC References
    Epitope Map References
    All References

    Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
    Gomez-Sanchez EP
    Endocrinology 147.3 (2006 Mar): 1343-8.

    Localisation of 11β-Hydroxysteroid Dehydrogenase Type 2 in Mineralocorticoid Receptor Expressing Magnocellular Neurosecretory Neurones of the Rat Supraoptic and Paraventricular Nuclei.
    Teruyama R
    Journal of neuroendocrinology 27.11 (2015 Nov): 835-49.

    Immunohistochemical demonstration of the mineralocorticoid receptor, 11beta-hydroxysteroid dehydrogenase-1 and -2, and hexose-6-phosphate dehydrogenase in rat ovary.
    Gomez-Sanchez CE
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.7 (2009 Jul): 633-41.

    Epithelial Na⁺ sodium channels in magnocellular cells of the rat supraoptic and paraventricular nuclei.
    Armstrong WE
    American journal of physiology. Endocrinology and metabolism 302.3 (2012 Feb 1): E273-85.

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