MRN3 3F10

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$45.00 to $425.00
SKU: MRN3 3F10
View product citations for antibody MRN3 3F10 on CiteAb

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Available: 28

DSHB Data Sheet

Catalog Fields

Antigen: mineralocorticoid receptor
Available to For-Profits: Yes
Antigen Species: Rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Gomez-Sanchez, C.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Mouse, Rat
Depositors Institution: G.V. Montgomery VA Medical Center
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Depositors Notes: Doesn't work for western blotting.
Predicted Species Reactivity:  
Human Protein Atlas:  
Gene: Nr3c2
Alternate Antibody Name: rMR 79–97 3F10
Alternate Gene Names: Mlr, MCR
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2
Epitope Mapped: Yes
Uniprot ID: P22199 
Epitope Location or Sequence: N-terminus A/B domain (aa 79-97)
Entrez Gene ID: 25672 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_1157908 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information: Should recognize all MR isoforms. The sequence of the rMR 79–97 peptide is identical between rat and mouse, but differs in human.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MRN3 3F10 was deposited to the DSHB by Gomez-Sanchez, C. (DSHB Hybridoma Product MRN3 3F10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IHC References
    Epitope Map References
    All References

    Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
    Gomez-Sanchez EP
    Endocrinology 147.3 (2006 Mar): 1343-8.

    Localisation of 11β-Hydroxysteroid Dehydrogenase Type 2 in Mineralocorticoid Receptor Expressing Magnocellular Neurosecretory Neurones of the Rat Supraoptic and Paraventricular Nuclei.
    Teruyama R
    Journal of neuroendocrinology 27.11 (2015 Nov): 835-49.

    Immunohistochemical demonstration of the mineralocorticoid receptor, 11beta-hydroxysteroid dehydrogenase-1 and -2, and hexose-6-phosphate dehydrogenase in rat ovary.
    Gomez-Sanchez CE
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.7 (2009 Jul): 633-41.

    Epithelial Na⁺ sodium channels in magnocellular cells of the rat supraoptic and paraventricular nuclei.
    Armstrong WE
    American journal of physiology. Endocrinology and metabolism 302.3 (2012 Feb 1): E273-85.

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