MS13

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$40.00
SKU: MS13
View product citations for antibody MS13 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: hemocytes, moth larvae
Hybridoma Cells Available: Yes
Antigen Species: Manduca sexta
Depositor: Kanost, M.R.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Kansas State University
Positive Tested Species Reactivity: Helicoverpa zea, Manduca sexta
Depositors Notes: Antigen note: beta integrin. Specificity note: Manduca sexta, also labels granular hemocytes from Helicoverpa zea. Functional effects: block plasmatocyte spreading and encapsulation; binds to neuroglian and a tetraspanin.
Antigen Molecular Weight: 90 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: hemocytes from larvae of Manduca sexta, the tobacco hornworm; whole cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: X63Ag8.653
Epitope Location or Sequence: see Levin et al. (2005) (PMID 15804572)
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MS13 was deposited to the DSHB by Kanost, M.R. (DSHB Hybridoma Product MS13)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • WB References
  • All References
  • Initial Publication
    WB References

    A hemocyte-specific integrin required for hemocytic encapsulation in the tobacco hornworm, Manduca sexta.
    Kanost MR
    Insect biochemistry and molecular biology 35.5 (2005 May): 369-80.

    All References

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