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DSHB Data Sheet

Catalog Fields

Product Name/ID: MS13
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_2618094 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: hemocytes from larvae of Manduca sexta, the tobacco hornworm; whole cells
Immunogen Sequence:
Myeloma Strain: X63Ag8.653
Epitope Mapped: No
Antigen Name: hemocytes, moth larvae
Epitope Location or Sequence: see Levin et al. (2005) (PMID 15804572)
Alternate Antigen Name:
Deposit Date: 7/27/2011
Antigen Molecular Weight: 90 kDa
Depositor: Kanost, M.R.
Antigen Sequence:
Depositor Institution: Kansas State University
Antigen Species: Manduca sexta
Depositor Notes: Antigen note: beta integrin. Specificity note: Manduca sexta, also labels granular hemocytes from Helicoverpa zea. Functional effects: block plasmatocyte spreading and encapsulation; binds to neuroglian and a tetraspanin.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Helicoverpa zea, Manduca sexta
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MS13 was deposited to the DSHB by Kanost, M.R. (DSHB Hybridoma Product MS13)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • WB References
  • All References
  • Initial Publication
    WB References

    A hemocyte-specific integrin required for hemocytic encapsulation in the tobacco hornworm, Manduca sexta.
    Kanost MR
    Insect biochemistry and molecular biology 35.5 (2005 May): 369-80.

    All References

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