anti-PSMA [muJ591]
Catalog Fields
Clone ID/Product Name: anti-PSMA [muJ591]
Available to For-Profits: No
Alternate Antibody Name: J591
Gene Symbol: FOLH1
Ab Isotype: MIgG1
Gene Name:
Uniprot ID: Q04609
Entrez Gene ID: 2346
Clonality: Monoclonal
Immunogen: LNCaP cell lysate and/or primary PCa epithelial cells
Clone:
Immunogen Sequence: Full-length
Myeloma Strain: X63.Ag.653
Epitope Mapped: Yes
Antigen Name: PSMA/FOLH1
Epitope Location or Sequence: extracellular domain of PSMA, apical region
Alternate Antigen Name: Prostate specific membrane antigen; folate hydrolase 1.
Deposit Date: 6/30/2023
Antigen Molecular Weight: 100 kDa
Depositor: Bander, N.H.
Antigen Sequence:
Depositor Institution: Weill Cornell Medicine
Antigen Species: Human
Depositor Notes: MUJ591 has a binding affinity (Kd) of 1.83 nM. PMID: 11016653.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: No fixation. IHC and IF on frozen sections only.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
anti-PSMA [muJ591] was deposited to the DSHB by Bander, N.H. (DSHB Hybridoma Product anti-PSMA [muJ591])
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
1 Reference
Initial Publication
Monoclonal antibodies to the extracellular domain of prostate-specific membrane antigen also react with tumor vascular endothelium.
Bander NH
Cancer research 57.17 (1997 Sep 1): 3629-34.