Mab 4860

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$45.00 to $425.00
SKU: Mab 4860
View product citations for antibody Mab 4860 on CiteAb

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Available: 7

DSHB Data Sheet

Catalog Fields

Antigen: Oligodendrocyte glycolipid marker
Available to For-Profits: Yes
Antigen Species: Rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: RIgM
Depositor: Faissner, Andreas
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Chicken, Human, Mouse
Depositors Institution: Ruhr University, Bochum, Germany
Antigen Molecular Weight:
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Rest-L2 glycoprotein fraction from mouse brain
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: X-Ag8-653
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_2890102 
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry
Additional Information: An affinity-purified anti-rat IgM heavy chain secondary antibody is strongly recommended for the best results.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 4860 was deposited to the DSHB by Faissner, Andreas (DSHB Hybridoma Product Mab 4860)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Novel conserved oligodendrocyte surface epitope identified by monoclonal antibody 4860.
    Faissner A
    Cell and tissue research 338.2 (2009 Nov): 161-70.

    IF References
    WB References
    IHC References
    ELISA References

    Novel conserved oligodendrocyte surface epitope identified by monoclonal antibody 4860.
    Faissner A
    Cell and tissue research 338.2 (2009 Nov): 161-70.

    Epitope Map References

    LewisX: a neural stem cell specific glycan?
    Faissner A
    The international journal of biochemistry & cell biology 44.6 (2012 Jun): 830-3.

    All References

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