Mab 5750Lex

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$45.00
SKU: Mab 5750Lex
View product citations for antibody Mab 5750Lex on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: LewisX-type carbohydrates
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Faissner, Andreas
Isotype: RIgM
Antigen Sequence:
Host Species: rat
Depositors Institution: Ruhr University, Bochum, Germany
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: The epitope recognized by this antibody is found on DSD-1-PG/phosphacan, L1-CAM and tenascin-C.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Purified Tenascin-C from mouse brain
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: X-Ag8-653
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: Full length sequence
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2890103 
Additional Information: An affinity-purified anti-rat IgM heavy chain secondary antibody is strongly recommended for the best results. 22433989
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 5750Lex was deposited to the DSHB by Faissner, Andreas (DSHB Hybridoma Product Mab 5750Lex)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells.
    Faissner A
    The Journal of biological chemistry 286.18 (2011 May 6): 16321-31.

    IF References
    WB References
    IHC References
    IP References
    ELISA References

    Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells.
    Faissner A
    The Journal of biological chemistry 286.18 (2011 May 6): 16321-31.

    FB References
    FACS References

    Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells.
    Faissner A
    The Journal of biological chemistry 286.18 (2011 May 6): 16321-31.

    Epitope Map References

    LewisX: a neural stem cell specific glycan?
    Faissner A
    The international journal of biochemistry & cell biology 44.6 (2012 Jun): 830-3.

    All References

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