Mab 5750Lex
Catalog Fields
Clone ID/Product Name: Mab 5750Lex
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: RIgM
Gene Name:
Antibody Registry ID: AB_2890103
Clonality: Monoclonal
Immunogen: Purified Tenascin-C from mouse brain
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: X-Ag8-653
Epitope Mapped: No
Antigen Name: LewisX-type carbohydrates
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/17/2021
Antigen Molecular Weight:
Depositor: Faissner, Andreas
Antigen Sequence:
Depositor Institution: Ruhr University, Bochum, Germany
Antigen Species: Mouse
Depositor Notes: The epitope recognized by this antibody is found on DSD-1-PG/phosphacan, L1-CAM and tenascin-C.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: An affinity-purified anti-rat IgM heavy chain secondary antibody is strongly recommended for the best results. 22433989
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 5750Lex was deposited to the DSHB by Faissner, Andreas (DSHB Hybridoma Product Mab 5750Lex)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
8 References
Initial Publication
Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells.
Faissner A
The Journal of biological chemistry 286.18 (2011 May 6): 16321-31.