Mab 5750Lex

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$45.00 to $425.00
SKU: Mab 5750Lex
View product citations for antibody Mab 5750Lex on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: LewisX-type carbohydrates
Available to For-Profits: Yes
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Isotype: RIgM
Depositor: Faissner, Andreas
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse
Depositors Institution: Ruhr University, Bochum, Germany
Antigen Molecular Weight:
Depositors Notes: The epitope recognized by this antibody is found on DSD-1-PG/phosphacan, L1-CAM and tenascin-C.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Purified Tenascin-C from mouse brain
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: X-Ag8-653
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_2890103 
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: An affinity-purified anti-rat IgM heavy chain secondary antibody is strongly recommended for the best results. 22433989
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 5750Lex was deposited to the DSHB by Faissner, Andreas (DSHB Hybridoma Product Mab 5750Lex)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells.
    Faissner A
    The Journal of biological chemistry 286.18 (2011 May 6): 16321-31.

    IF References
    WB References
    IHC References
    IP References
    ELISA References

    Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells.
    Faissner A
    The Journal of biological chemistry 286.18 (2011 May 6): 16321-31.

    FB References
    FACS References

    Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells.
    Faissner A
    The Journal of biological chemistry 286.18 (2011 May 6): 16321-31.

    Epitope Map References

    LewisX: a neural stem cell specific glycan?
    Faissner A
    The international journal of biochemistry & cell biology 44.6 (2012 Jun): 830-3.

    All References

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