Mef2

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$45.00
SKU: Mef2-p
View product citations for antibody Mef2 on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: Myocyte-specific enhancer factor 2
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): No
Isotype: Rabbit IgG
Depositor: Nguyen, HT
Host Species: rabbit
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Universitaet Erlangen- Nuernberg
Antigen Molecular Weight: 57.0 kDa
Depositors Notes: Recommended dilution 1:1000
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: GEX2T-MEF2 fusion protein
Gene: Mef2
Alternate Antibody Name:
Alternate Gene Names: CG1429
Alternate Antigen Name:
Clonality: Polyclonal
Myeloma Strain: NA
Epitope Mapped: No
Uniprot ID: P40791 
Epitope Location or Sequence:
Entrez Gene ID: 36032 
Immunogen Sequence: a.a. 1-168
Antibody Registry ID: AB_2892602 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information: FBrf0227968
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mef2 was deposited to the DSHB by Nguyen, HT (DSHB Hybridoma Product Mef2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Drosophila MEF2, a transcription factor that is essential for myogenesis.
    Nguyen HT
    Genes & development 9.6 (1995 Mar 15): 730-41.

    IF References

    Drosophila Lame duck, a novel member of the Gli superfamily, acts as a key regulator of myogenesis by controlling fusion-competent myoblast development.
    Nguyen HT
    Development (Cambridge, England) 128.22 (2001 Nov): 4489-500.

    The transcription factor Mef2 is required for normal circadian behavior in Drosophila.
    Blau J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.17 (2010 Apr 28): 5855-65.

    IHC References
    All References

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