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SKU: Mef2-p
View product citations for antibody Mef2 on CiteAb

In Stock

Available: 89

DSHB Data Sheet

Catalog Fields

Antigen: Myocyte-specific enhancer factor 2
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Nguyen, HT
Isotype: Rabbit IgG
Antigen Sequence:
Host Species: rabbit
Depositors Institution: Universitaet Erlangen- Nuernberg
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Recommended dilution 1:1000
Antigen Molecular Weight: 57.0 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Mef2
Immunogen: GEX2T-MEF2 fusion protein
Alternate Gene Names: CG1429
Alternate Antibody Name:
Clonality: Polyclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NA
Epitope Location or Sequence:
Uniprot ID: P40791 
Immunogen Sequence: a.a. 1-168
Entrez Gene ID: 36032 
Additional Characterization:  
Antibody Registry ID: AB_2892602 
Additional Information: FBrf0227968
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mef2 was deposited to the DSHB by Nguyen, HT (DSHB Hybridoma Product Mef2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Drosophila MEF2, a transcription factor that is essential for myogenesis.
    Nguyen HT
    Genes & development 9.6 (1995 Mar 15): 730-41.

    IF References

    Drosophila Lame duck, a novel member of the Gli superfamily, acts as a key regulator of myogenesis by controlling fusion-competent myoblast development.
    Nguyen HT
    Development (Cambridge, England) 128.22 (2001 Nov): 4489-500.

    The transcription factor Mef2 is required for normal circadian behavior in Drosophila.
    Blau J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.17 (2010 Apr 28): 5855-65.

    IHC References
    All References

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