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DSHB Data Sheet

Catalog Fields

Antigen: melanoblasts and melanocytes of neural crest origin (avian) / glutathione S-transferase (alpha subunit)
Hybridoma Cells Available: Yes
Antigen Species: quail
Depositor: Le Douarin, N.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Institut d’Embryologie Cellulaire et Moleculaire du C.N.R.S. et du College de France
Positive Tested Species Reactivity: Avian
Depositors Notes: Cell binding note: melanoblasts and melanocytes of neural crest origin. Immunoblotting note: yes, if protein is purified. IHC note: cytoplasmic antigen.
Antigen Molecular Weight: 26 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: preparation of membranes obtained from quail trunk neural crest cells in culture
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531849 
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MelEM was deposited to the DSHB by Le Douarin, N. (DSHB Hybridoma Product MelEM)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Novel markers of melanocyte differentiation in the avian embryo.
    Le Douarin NM
    Experimental cell research 207.1 (1993 Jul): 171-82.

    All References

    Novel markers of melanocyte differentiation in the avian embryo.
    Le Douarin NM
    Experimental cell research 207.1 (1993 Jul): 171-82.

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