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DSHB Data Sheet

Catalog Fields

Product Name/ID: MelEM
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_531849 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: preparation of membranes obtained from quail trunk neural crest cells in culture
Immunogen Sequence:
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: melanoblasts and melanocytes of neural crest origin (avian) / glutathione S-transferase (alpha subunit)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/12/1994
Antigen Molecular Weight: 26 kDa
Depositor: Le Douarin, N.
Antigen Sequence:
Depositor Institution: Institut d’Embryologie Cellulaire et Moleculaire du C.N.R.S. et du College de France
Antigen Species: quail
Depositor Notes: Cell binding note: melanoblasts and melanocytes of neural crest origin. Immunoblotting note: yes, if protein is purified. IHC note: cytoplasmic antigen.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MelEM was deposited to the DSHB by Le Douarin, N. (DSHB Hybridoma Product MelEM)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Novel markers of melanocyte differentiation in the avian embryo.
    Le Douarin NM
    Experimental cell research 207.1 (1993 Jul): 171-82.

    All References

    Novel markers of melanocyte differentiation in the avian embryo.
    Le Douarin NM
    Experimental cell research 207.1 (1993 Jul): 171-82.

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