N114/10

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$45.00
SKU: N114/10
View product citations for antibody N114/10 on CiteAb

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Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Trimmer, J.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: UC - Davis
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Does not cross-react with other HCN’s.
Antigen Molecular Weight: 120-140 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: HCN4
Immunogen: Fusion Protein
Alternate Gene Names: BRGDA8, EIG18, SSS2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence: Cytoplasmic C-terminus)
Uniprot ID: Q9Y3Q4 
Immunogen Sequence: a.a.1021-1110
Entrez Gene ID: 10021 
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N114/10 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N114/10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Countervailing modulation of Ih by neuropeptide Y and corticotrophin-releasing factor in basolateral amygdala as a possible mechanism for their effects on stress-related behaviors.
    Colmers WF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.50 (2010 Dec 15): 16970-82.

    All References

    Countervailing modulation of Ih by neuropeptide Y and corticotrophin-releasing factor in basolateral amygdala as a possible mechanism for their effects on stress-related behaviors.
    Colmers WF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.50 (2010 Dec 15): 16970-82.

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