N133/21

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$45.00
SKU: N133/21
View product citations for antibody N133/21 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Regulator of G-protein signaling 14 (RGS14)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Trimmer, J.S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: UC - Davis
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: 60 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Rgs14
Immunogen: Fusion Protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 444-490
Uniprot ID: O08773 
Immunogen Sequence: a.a. 1-544 (full length senece)
Entrez Gene ID: 114705 
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N133/21 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N133/21)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Epitope Map References
  • All References
  • Epitope Map References

    Postnatal developmental expression of regulator of G protein signaling 14 (RGS14) in the mouse brain.
    Hepler JR
    The Journal of comparative neurology 522.1 (2014 Jan 1): 186-203.

    All References

    Postnatal developmental expression of regulator of G protein signaling 14 (RGS14) in the mouse brain.
    Hepler JR
    The Journal of comparative neurology 522.1 (2014 Jan 1): 186-203.

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