Product Name/ID: N144/14
Available to For-Profits: Yes
Alternate Antibody Name:
Ab Isotype: MIgG1
Immunogen: Fusion protein
Immunogen Sequence: 6XHistidine
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Hexahistidine (6xHis)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/10/2021
Antigen Molecular Weight:
Depositor: Trimmer, J.S.
Depositor Institution: UC - Davis
Antigen Species: N.A.
Depositor Notes: This antibody was identified as an off-target Ab in a screen for anti-Protocadherin Gamma A3 and recognizes native protein in dot blots.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: N.A.
Additional Information: The fusion protein immunogen used to generate this hybridoma was provided by Dr. Joshua Weiner at the University of Iowa.
Additional Characterization: https://neuromab.ucdavis.edu/datasheet/N144_14.pdf
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N144/14 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N144/14)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.