N159/5
Catalog Fields
Clone ID/Product Name: N159/5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: PCDH
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2877195
Clonality: Monoclonal
Immunogen: Fusion protein
Clone:
Immunogen Sequence: a.a 808-931
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Pan-Gamma-protocadherin-Constant
Epitope Location or Sequence: Constant region at the cytoplasmic C-terminus
Alternate Antigen Name:
Deposit Date: 11/10/2021
Antigen Molecular Weight: 100 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Mouse
Depositor Notes: This antibody recognizes the cytoplasmic C-terminal constant domain shared by all gamma-protocadherins (A, B, and C subfamilies).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information: Cross-reacts with all Gamma-
protocadherin-A, -B and -C proteins. This antibody has been validated in PCDH KO mice.
protocadherin-A, -B and -C proteins. This antibody has been validated in PCDH KO mice.
Additional Characterization: https://neuromab.ucdavis.edu/datasheet/N159_5.pdf
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N159/5 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N159/5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
13 References
Initial Publication
Control of CNS synapse development by {gamma}-protocadherin-mediated astrocyte-neuron contact.
Weiner JA
The Journal of neuroscience : the official journal of the Society for Neuroscience 29.38 (2009 Sep 23): 11723-31.