N295B/66

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$45.00
SKU: N295B/66
View product citations for antibody N295B/66 on CiteAb

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Available: 34

DSHB Data Sheet

Catalog Fields

Antigen: ADP-ribosylation factor-like protein 13B
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Trimmer, J.S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: UC - Davis
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: Arl13b is a cilium-specific protein required for ciliary axoneme structure.
Antigen Molecular Weight: 60 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Arl13b
Immunogen: Fusion Protein
Alternate Gene Names: Arl2l1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q640N2 
Immunogen Sequence: a.a. 208-427
Entrez Gene ID: 68146 
Antibody Registry ID:  
Additional Information: N295B/66 specificity has been validated in null MEF cells.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N295B/66 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N295B/66)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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