N325B/65
Catalog Fields
Clone ID/Product Name: N325B/65
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: THAP1
Ab Isotype: MIgG3
Gene Name:
Antibody Registry ID: AB_2877553
Uniprot ID: Q9NVV9
Entrez Gene ID: 55145
Clonality: Monoclonal
Immunogen: Fusion protein amino acids 1-213 of human THAP1
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: THAP1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/28/2023
Antigen Molecular Weight: 30 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: human
Depositor Notes: No cross-reactivity with THAP2, THAP3, and other THAP proteins
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity:
Additional Information:
Predicted Species Reactivity:
Human Protein Atlas: https://www.proteinatlas.org/ENSG00000131931-THAP1
Additional Characterization: https://neuromab.ucdavis.edu/datasheet/N325B_65.pdf
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N325B/65 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N325B/65)
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
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