N327/95
Catalog Fields
Antigen: Glutamate receptor ionotropic, NMDA 2A
Available to For-Profits: Yes
Antigen Species: Rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a
Depositor: Trimmer, J.S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Institution: UC - Davis
Antigen Molecular Weight: 170 kDa
Depositors Notes: Does not cross-react with NR2B
Immunogen: Fusion Protein
Gene: Grin2a
Alternate Antibody Name:
Alternate Gene Names: GluN2A, NMDAR2A, NR2A
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: Q00959
Epitope Location or Sequence: extracellular N-terminus
Entrez Gene ID: 24409
Immunogen Sequence: a.a. 75-325
Additional Characterization: https://neuromab.ucdavis.edu/datasheet/N327_95.pdf
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N327/95 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N327/95)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
1 Reference
Initial Publication
CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons.
Broccoli V
Nature cell biology 14.9 (2012 Sep): 911-23.