N377/20

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$45.00 to $425.00
SKU: N377/20
View product citations for antibody N377/20 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Mitogen-activated protein kinase kinase kinase 12
Available to For-Profits: Yes
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a
Depositor: Trimmer, J.S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Mouse, Rat
Depositors Institution: UC - Davis
Antigen Molecular Weight: 120 kda
Depositors Notes: Does not cross-react with MAP3K13.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Fusion Protein
Gene: MAP3K12
Alternate Antibody Name:
Alternate Gene Names: DL, DLK, MUK, Zp, Zpk
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: Q60700 
Epitope Location or Sequence: C-terminus
Entrez Gene ID: 26404 
Immunogen Sequence: a.a. 727-888
Antibody Registry ID:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N377/20 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N377/20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    HSP90 is a chaperone for DLK and is required for axon injury signaling.
    DiAntonio A
    Proceedings of the National Academy of Sciences of the United States of America 115.42 (2018 Oct 16): E9899-E9908.

    All References

    HSP90 is a chaperone for DLK and is required for axon injury signaling.
    DiAntonio A
    Proceedings of the National Academy of Sciences of the United States of America 115.42 (2018 Oct 16): E9899-E9908.

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