N419/40

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SKU: N419/40

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: N419/40
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: SCN1A
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_2877589 
Uniprot ID: P35498 
RRID:  
Entrez Gene ID: 6323 
Clonality: Monoclonal
Immunogen: Synthetic peptide
Clone:
Immunogen Sequence: a.a. 1501-1518
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Pan-Nav1 sodium channel
Epitope Location or Sequence: cytoplasmic loop between repeat III and IV
Alternate Antigen Name:
Deposit Date: 11/10/2021
Antigen Molecular Weight: 228.9 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Human
Depositor Notes: Cross-reacts with multiple Nav1 channels. use in lieu of K58/35 IgG1 for additional multiplex labeling combinations.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N419/40 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N419/40)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments.
    Sadakata T
    The Journal of neuroscience : the official journal of the Society for Neuroscience 39.32 (2019 Aug 7): 6339-6353.

    All References

    Deletion of Class II ADP-Ribosylation Factors in Mice Causes Tremor by the Nav1.6 Loss in Cerebellar Purkinje Cell Axon Initial Segments.
    Sadakata T
    The Journal of neuroscience : the official journal of the Society for Neuroscience 39.32 (2019 Aug 7): 6339-6353.

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