N501/49
Catalog Fields
Clone ID/Product Name: N501/49
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Ryr2
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_3674643
Uniprot ID: E9Q401
Entrez Gene ID: 20191
Clonality: Monoclonal
Immunogen: Recombinant protein amino acids 2672-2835 (cytoplasmic) of mouse Ryanodine Receptor Type 2
Clone:
Immunogen Sequence:
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: RyR2
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/16/2025
Antigen Molecular Weight:
Depositor: UC Davis/NIH NeuroMab Facility
Antigen Sequence:
Depositor Institution: UC Davis
Antigen Species: mouse
Depositor Notes:
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity:
Additional Information:
Additional Characterization: https://neuromab.ucdavis.edu/datasheet/N500_49.pdf
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N501/49 was deposited to the DSHB by UC Davis/NIH NeuroMab Facility (DSHB Hybridoma Product N501/49)
Storage and Handling Recommendations
Although many cell products are maintained at 4ûC for years without loss of activity, shelf-life at 4ûC is highly variable. For immediate use, short term storage at 4ûC up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20ûC or -80ûC. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
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