N59/20

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$45.00
SKU: N59/20
View product citations for antibody N59/20 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: GluN2B/NR2B glutamate receptor
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Trimmer, J.S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: UC - Davis
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: This antigen is also known as Glutamate receptor ionotropic, NMDA 2B..
Antigen Molecular Weight: 170 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Grin2b
Immunogen: Fusion Protein
Alternate Gene Names: GluN2B
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence: Extracellular N-terminus
Uniprot ID: Q00960 
Immunogen Sequence: a.a. 20-271
Entrez Gene ID: 24410 
Antibody Registry ID:  
Additional Information: Does not cross-react with GluN2A/NR2A, GluN2C/NR2C or
GluN2D/NR2D (based on KO validation results).
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N59/20 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N59/20)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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