N67/15

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SKU: N67/15

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DSHB Data Sheet

Catalog Fields

Product Name/ID: N67/15
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: TRPC5
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID:  
Uniprot ID: Q9UL62 
RRID:  
Entrez Gene ID: 7224 
Clonality: Monoclonal
Immunogen: Synthetic peptide
Clone:
Immunogen Sequence: a.a. 827-845
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: TrpC5 calcium channel,
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/10/2021
Antigen Molecular Weight: 111.4 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Human
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N67/15 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N67/15)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Hypoosmotic- and pressure-induced membrane stretch activate TRPC5 channels.
    Viana F
    The Journal of physiology 586.23 (2008 Dec 1): 5633-49.

    All References

    Hypoosmotic- and pressure-induced membrane stretch activate TRPC5 channels.
    Viana F
    The Journal of physiology 586.23 (2008 Dec 1): 5633-49.

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