N69/46

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SKU: N69/46

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DSHB Data Sheet

Catalog Fields

Product Name/ID: N69/46
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Shank3
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID:  
Uniprot ID: Q9JLU4 
RRID:  
Entrez Gene ID: 59312 
Clonality: Monoclonal
Immunogen: Synthetic peptide
Clone:
Immunogen Sequence: a.a. 840-857
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Shank3
Epitope Location or Sequence: PEKLPGSLRKGIPRTKSV
Alternate Antigen Name:
Deposit Date: 6/7/2022
Antigen Molecular Weight: 190 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Rat
Depositor Notes: No cross-reactivity against Shank1 or Shank2.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N69/46 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N69/46)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Kalirin-7 is required for synaptic structure and function.
    Mains RE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.47 (2008 Nov 19): 12368-82.

    All References

    Kalirin-7 is required for synaptic structure and function.
    Mains RE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.47 (2008 Nov 19): 12368-82.

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