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$45.00 to $425.00
SKU: N74/25
View product citations for antibody N74/25 on CiteAb

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Available: 9

DSHB Data Sheet

Catalog Fields

Antigen: TrpM7 ion channel and Ser/Thr kinase,
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Trimmer, J.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: UC - Davis
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: Not yet validated for use in mammalian brain. No cross-reactivity with TrpM6.
Antigen Molecular Weight: 212.3 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Trpm7
Immunogen: Fusion protein
Alternate Gene Names: CHAK, CHAK1, LTrpC-7, Ltpr7, Ltrpc7, TRPPLIK
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence: Cytoplasmic C-terminus
Uniprot ID: Q923J1 
Immunogen Sequence: a.a. 1817-1863
Entrez Gene ID: 58800 
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N74/25 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N74/25)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    TRPM7-mediated Ca2+ signals confer fibrogenesis in human atrial fibrillation.
    Yue L
    Circulation research 106.5 (2010 Mar 19): 992-1003.

    All References

    TRPM7-mediated Ca2+ signals confer fibrogenesis in human atrial fibrillation.
    Yue L
    Circulation research 106.5 (2010 Mar 19): 992-1003.

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