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$45.00 to $425.00
SKU: N74/25
View product citations for antibody N74/25 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Product Name/ID: N74/25
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Trpm7
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID:  
Uniprot ID: Q923J1 
Entrez Gene ID: 58800 
Clonality: Monoclonal
Immunogen: Fusion protein
Immunogen Sequence: a.a. 1817-1863
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: TrpM7 ion channel and Ser/Thr kinase,
Epitope Location or Sequence: Cytoplasmic C-terminus
Alternate Antigen Name:
Deposit Date: 11/10/2021
Antigen Molecular Weight: 212.3 kDa
Depositor: Trimmer, J.S.
Antigen Sequence:
Depositor Institution: UC - Davis
Antigen Species: Mouse
Depositor Notes: Not yet validated for use in mammalian brain. No cross-reactivity with TrpM6.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Recommended Applications: Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N74/25 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N74/25)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    TRPM7-mediated Ca2+ signals confer fibrogenesis in human atrial fibrillation.
    Yue L
    Circulation research 106.5 (2010 Mar 19): 992-1003.

    All References

    TRPM7-mediated Ca2+ signals confer fibrogenesis in human atrial fibrillation.
    Yue L
    Circulation research 106.5 (2010 Mar 19): 992-1003.

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