N77/15

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$45.00 to $425.00
SKU: N77/15
View product citations for antibody N77/15 on CiteAb

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Available: 9

DSHB Data Sheet

Catalog Fields

Antigen: TrpC4
Available to For-Profits: Yes
Antigen Species: Rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2b
Depositor: Trimmer, J.S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Institution: UC - Davis
Antigen Molecular Weight: 110 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Fusion protein
Gene: Trpc4
Alternate Antibody Name:
Alternate Gene Names: Trp4, Trrp4
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Uniprot ID: O35119 
Epitope Location or Sequence: FKSEKVVVEDTVPIIPKE
Entrez Gene ID: 84494 
Immunogen Sequence: a.a. 930-947
Antibody Registry ID:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N77/15 was deposited to the DSHB by Trimmer, J.S. (DSHB Hybridoma Product N77/15)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

1 Reference

  • Initial Publication
  • IP References
  • All References
  • Initial Publication

    Mass spectrometric analysis of novel phosphorylation sites in the TRPC4β channel.
    Park KS
    Rapid communications in mass spectrometry : RCM 26.17 (2012 Sep 15): 1965-70.

    IP References

    Mass spectrometric analysis of novel phosphorylation sites in the TRPC4β channel.
    Park KS
    Rapid communications in mass spectrometry : RCM 26.17 (2012 Sep 15): 1965-70.

    All References

    Mass spectrometric analysis of novel phosphorylation sites in the TRPC4β channel.
    Park KS
    Rapid communications in mass spectrometry : RCM 26.17 (2012 Sep 15): 1965-70.

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