NELF-E

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$50.00
SKU: NELF-E-p

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Available: 26

DSHB Data Sheet

Catalog Fields

Product Name/ID: NELF-E
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: Nelf-E
Ab Isotype: Rabbit IgG
Gene Symbol:
Antibody Registry ID: AB_2938696 
Uniprot ID: P92204 
RRID:  
Entrez Gene ID: 38982 
Clonality: Polyclonal
Immunogen: His-tag fusion protein
Clone:
Immunogen Sequence: Full-length
Myeloma Strain: N.A.
Epitope Mapped: No
Antigen Name: Negative elongation factor E
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/18/2023
Antigen Molecular Weight: 31.8 kDa
Depositor: Gilmour, D.S.
Antigen Sequence:
Depositor Institution: Penn State Univ.
Antigen Species: Drosophila
Depositor Notes: This antisera is good for immunofluorescence staining polytene chromosomes. An initial dilution of 1:200 is recommended.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization: https://flybase.org/reports/FBgn0017430 
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
NELF-E was deposited to the DSHB by Gilmour, D.S. (DSHB Hybridoma Product NELF-E)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

3 References

  • Initial Publication
  • WB References
  • ChIP References
  • Microarray References
  • All References
  • Initial Publication

    NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila.
    Gilmour D
    Genes & development 17.11 (2003 Jun 1): 1402-14.

    WB References

    Molecular characterization of Drosophila NELF.
    Gilmour DS
    Nucleic acids research 33.4 (2005): 1269-79.

    NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila.
    Gilmour D
    Genes & development 17.11 (2003 Jun 1): 1402-14.

    ChIP References

    Molecular characterization of Drosophila NELF.
    Gilmour DS
    Nucleic acids research 33.4 (2005): 1269-79.

    NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila.
    Gilmour D
    Genes & development 17.11 (2003 Jun 1): 1402-14.

    Microarray References

    NELF and GAGA factor are linked to promoter-proximal pausing at many genes in Drosophila.
    Gilmour DS
    Molecular and cellular biology 28.10 (2008 May): 3290-300.

    All References

    Molecular characterization of Drosophila NELF.
    Gilmour DS
    Nucleic acids research 33.4 (2005): 1269-79.

    NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila.
    Gilmour D
    Genes & development 17.11 (2003 Jun 1): 1402-14.

    NELF and GAGA factor are linked to promoter-proximal pausing at many genes in Drosophila.
    Gilmour DS
    Molecular and cellular biology 28.10 (2008 May): 3290-300.

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