Catalog Fields
Antigen: Peptidoglycan-associated lipoprotein, PAL /P6
Hybridoma Cells Available: Yes
Antigen Species: Haemophilus influenzae
Depositor: Apicella, M.A.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: E. coli, Haemophilus influenzae, Salmonella typhimurium
Depositors Notes: NTHi 3B9 cross-reacts with E. coli OmpA and Salmonella typhimurium OmpA.
Predicted Species Reactivity:
Gene: pal
Immunogen: nontypable H. influenzae 2019 cells
Alternate Gene Names: ompP6, HI_0381
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8
Epitope Location or Sequence: Surface conformation epitope including Arg-147and Arg-148
Uniprot ID: P10324
Immunogen Sequence: Full length protein
Entrez Gene ID: 949485
Antibody Registry ID: AB_2617195
Additional Information: RRID: AB_2617195
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
NTHi 3B9 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product NTHi 3B9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
7 References
Initial Publication
Mapping of a surface-exposed, conformational epitope of the P6 protein of Haemophilus influenzae.
Apicella MA
Infection and immunity 63.11 (1995 Nov): 4395-401.