NTHi 3B9

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$50.00
SKU: NTHi 3B9

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Available: 41

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: NTHi 3B9
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: pal
Ab Isotype: MIgG2a
Gene Name:
Antibody Registry ID: AB_2617195 
Uniprot ID: P10324 
RRID:  
Entrez Gene ID: 949485 
Clonality: Monoclonal
Immunogen: nontypable H. influenzae 2019 cells
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3x63Ag8
Epitope Mapped: Yes
Antigen Name: Peptidoglycan-associated lipoprotein, PAL /P6
Epitope Location or Sequence: Surface conformation epitope including Arg-147and Arg-148
Alternate Antigen Name:
Deposit Date: 3/28/2013
Antigen Molecular Weight: 16.6 kDa
Depositor: Apicella, M.A.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Haemophilus influenzae
Depositor Notes: NTHi 3B9 cross-reacts with E. coli OmpA and Salmonella typhimurium OmpA.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: E. coli, Haemophilus influenzae, Salmonella typhimurium
Additional Information: RRID: AB_2617195
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
NTHi 3B9 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product NTHi 3B9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Distribution of bacterial proteins in biofilms formed by non-typeable Haemophilus influenzae.
    St Geme JW 3rd
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.7 (2006 Jul): 829-42.

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    IHC References

    Distribution of bacterial proteins in biofilms formed by non-typeable Haemophilus influenzae.
    St Geme JW 3rd
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.7 (2006 Jul): 829-42.

    Nontypeable Haemophilus influenzae in the lower respiratory tract of patients with chronic bronchitis.
    Greenberg SB
    American journal of respiratory and critical care medicine 164.11 (2001 Dec 1): 2114-9.

    ELISA References
    FACS References

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    Epitope Map References
    All References

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