NTHi 3B9

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$40.00
SKU: NTHi 3B9
View product citations for antibody NTHi 3B9 on CiteAb

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Available: 180

DSHB Data Sheet

Catalog Fields

Antigen: Peptidoglycan-associated lipoprotein, PAL /P6
Hybridoma Cells Available: Yes
Antigen Species: Haemophilus influenzae
Depositor: Apicella, M.A.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: E. coli, Haemophilus influenzae, Salmonella typhimurium
Depositors Notes: NTHi 3B9 cross-reacts with E. coli OmpA and Salmonella typhimurium OmpA.
Antigen Molecular Weight: 16.6 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: pal
Immunogen: nontypable H. influenzae 2019 cells
Alternate Gene Names: ompP6, HI_0381
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3x63Ag8
Epitope Location or Sequence: Surface conformation epitope including Arg-147and Arg-148
Uniprot ID: P10324 
Immunogen Sequence: Full length protein
Entrez Gene ID: 949485 
Additional Characterization:  
Antibody Registry ID: AB_2617195 
Additional Information: RRID: AB_2617195
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
NTHi 3B9 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product NTHi 3B9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Distribution of bacterial proteins in biofilms formed by non-typeable Haemophilus influenzae.
    St Geme JW 3rd
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.7 (2006 Jul): 829-42.

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    IHC References

    Distribution of bacterial proteins in biofilms formed by non-typeable Haemophilus influenzae.
    St Geme JW 3rd
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.7 (2006 Jul): 829-42.

    Nontypeable Haemophilus influenzae in the lower respiratory tract of patients with chronic bronchitis.
    Greenberg SB
    American journal of respiratory and critical care medicine 164.11 (2001 Dec 1): 2114-9.

    ELISA References
    FACS References

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    Epitope Map References
    All References

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