NTHi 7F3

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DSHB Data Sheet

Catalog Fields

Antigen: Peptidoglycan-associated lipoprotein, PAL /P6
Hybridoma Cells Available: Yes
Antigen Species: Haemophilus influenzae
Depositor: Apicella, M.A.
Isotype: MIgG3, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Haemophilus influenzae
Depositors Notes: Immunogen species is Haemophilus influenzae type b strain 2. NTHi 7F3 is highly specific for H. influenzae type b.
Antigen Molecular Weight: 16.6 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: pal
Immunogen: H. influenzae 3524 cells
Alternate Gene Names: ompP6, HI_0381
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: Surface conformational epitope including a.a. 59-61
Uniprot ID: P10324 
Immunogen Sequence: Full length protein
Entrez Gene ID: 949485 
Additional Characterization:  
Antibody Registry ID: AB_2617196 
Additional Information: RRID: AB_2617196
Recommended Applications: ELISA, FACS, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
NTHi 7F3 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product NTHi 7F3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Identification of a specific epitope of Haemophilus influenzae on a 16,600-dalton outer membrane protein.
    Apicella MA
    The Journal of infectious diseases 152.6 (1985 Dec): 1300-7.

    IF References

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    WB References
    ELISA References
    FACS References

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    Epitope Map References
    All References

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