NTHi 7F3

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$45.00 to $425.00
SKU: NTHi 7F3
View product citations for antibody NTHi 7F3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Peptidoglycan-associated lipoprotein, PAL /P6
Available to For-Profits: Yes
Antigen Species: Haemophilus influenzae
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG3, kappa light chain
Depositor: Apicella, M.A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Haemophilus influenzae
Depositors Institution: University of Iowa
Antigen Molecular Weight: 16.6 kDa
Depositors Notes: Immunogen species is Haemophilus influenzae type b strain 2. NTHi 7F3 is highly specific for H. influenzae type b.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: H. influenzae 3524 cells
Gene: pal
Alternate Antibody Name:
Alternate Gene Names: ompP6, HI_0381
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: Yes
Uniprot ID: P10324 
Epitope Location or Sequence: Surface conformational epitope including a.a. 59-61
Entrez Gene ID: 949485 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2617196 
Additional Characterization:  
Recommended Applications: ELISA, FACS, Immunofluorescence, Western Blot
Additional Information: RRID: AB_2617196
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
NTHi 7F3 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product NTHi 7F3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Identification of a specific epitope of Haemophilus influenzae on a 16,600-dalton outer membrane protein.
    Apicella MA
    The Journal of infectious diseases 152.6 (1985 Dec): 1300-7.

    IF References

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    WB References
    ELISA References
    FACS References

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    Epitope Map References
    All References

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