NTHi 7F3

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DSHB Data Sheet

Catalog Fields

Product Name/ID: NTHi 7F3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: pal
Ab Isotype: MIgG3, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2617196 
Uniprot ID: P10324 
Entrez Gene ID: 949485 
Clonality: Monoclonal
Immunogen: H. influenzae 3524 cells
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: Peptidoglycan-associated lipoprotein, PAL /P6
Epitope Location or Sequence: Surface conformational epitope including a.a. 59-61
Alternate Antigen Name:
Deposit Date: 3/28/2013
Antigen Molecular Weight: 16.6 kDa
Depositor: Apicella, M.A.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Haemophilus influenzae
Depositor Notes: Immunogen species is Haemophilus influenzae type b strain 2. NTHi 7F3 is highly specific for H. influenzae type b.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Haemophilus influenzae
Additional Information: RRID: AB_2617196
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
NTHi 7F3 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product NTHi 7F3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Identification of a specific epitope of Haemophilus influenzae on a 16,600-dalton outer membrane protein.
    Apicella MA
    The Journal of infectious diseases 152.6 (1985 Dec): 1300-7.

    IF References

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    WB References
    ELISA References
    FACS References

    Dual orientation of the outer membrane lipoprotein P6 of nontypeable haemophilus influenzae.
    Pichichero ME
    Journal of bacteriology 195.14 (2013 Jul): 3252-9.

    Epitope Map References
    All References

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