Occludin 5E5A6

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SKU: Occludin 5E5A6-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Occludin 5E5A6
Available to For-Profits: No
Alternate Antibody Name: Rat anti-occludin 5E5A6
Gene Name: OCLN
Ab Isotype: RIgG2a, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2819196 
Uniprot ID: Q16625 
RRID:  
Entrez Gene ID: 100506658 
Clonality: Monoclonal
Immunogen: Protein domain
Clone:
Immunogen Sequence: a.a. 272-522
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Occludin
Epitope Location or Sequence: The entire C-terminal cytoplasmic tail of human occludin with an S408A mutation.
Alternate Antigen Name:
Deposit Date: 1/27/2023
Antigen Molecular Weight: 59.1 kDa
Depositor: Turner, J.R.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Human
Depositor Notes: mAb 5E5A6 does not react with mouse or canine. Antibody is not selective for S408A or dephospho-S408 occludin.
Host Species: rat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: ELISA not tested / IF 1.0 ug/ml
WB 0.5 ug/ml / IHC-FFPE 1.0 ug/ml / IP not tested
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Occludin 5E5A6 was deposited to the DSHB by Turner, J.R. (DSHB Hybridoma Product Occludin 5E5A6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

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