Occludin 6B8A3
Catalog Fields
Product Name/ID: Occludin 6B8A3
Available to For-Profits: No
Alternate Antibody Name: Rat anti-occludin 6B8A3
Gene Name: OCLN
Ab Isotype: RIgG2a, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2819194
Uniprot ID: Q61146
Entrez Gene ID: 18260
Clonality: Monoclonal
Immunogen: Protein Domain
Clone:
Immunogen Sequence: a.a. 269-521
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Occludin
Epitope Location or Sequence: The entire C-terminal cytoplasmic tail of mouse occludin.
Alternate Antigen Name:
Deposit Date: 1/27/2023
Antigen Molecular Weight: 59.0 kDa
Depositor: Turner, J.R.
Antigen Sequence:
Depositor Institution: Harvard Medical School
Antigen Species: Mouse
Depositor Notes: Antibody is not selective for S494N occludin.
Host Species: rat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Canine, Human, Mouse
Additional Information: ELISA not tested / IF 1.0 ug/ml
WB 0.5 ug/ml / IHC-FFPE 1.0 ug/ml / IP not tested. Retrieval citrate buffer pH 6.0
WB 0.5 ug/ml / IHC-FFPE 1.0 ug/ml / IP not tested. Retrieval citrate buffer pH 6.0
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Occludin 6B8A3 was deposited to the DSHB by Turner, J.R. (DSHB Hybridoma Product Occludin 6B8A3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
1 Reference
Initial Publication
The Tight Junction Protein ZO-1 Is Dispensable for Barrier Function but Critical for Effective Mucosal Repair.
Turner JR
Gastroenterology 161.6 (2021 Dec): 1924-1939.