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SKU: Olig-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Olig
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_531795 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: CNS
Immunogen Sequence:
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: oligodendrocyte (myelin) marker (amphibian)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/29/1990
Antigen Molecular Weight:
Depositor: Constantine-Paton, M.
Antigen Sequence:
Depositor Institution: Massachusetts Institute of Technology
Antigen Species: frog
Depositor Notes: Cell binding: all embryonic and adult myelinated axon tracts. Does not work in goldfish and lamprey. IHC note: recognizes frog oligodendrocytes in paraformaldehyde fixed frozen sections, using Triton X-100; negative when used to stain tectal cell cultures or retinal axons
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: R. catesbeiana, R. pipiens, Xenopus
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Olig was deposited to the DSHB by Constantine-Paton, M. (DSHB Hybridoma Product Olig)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Monoclonal antibody markers for amphibian oligodendrocytes and neurons.
    Constantine-Paton M
    The Journal of comparative neurology 289.3 (1989 Nov 15): 467-80.

    All References

    Monoclonal antibody markers for amphibian oligodendrocytes and neurons.
    Constantine-Paton M
    The Journal of comparative neurology 289.3 (1989 Nov 15): 467-80.

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