P1F6
Catalog Fields
Clone ID/Product Name: P1F6
Available to For-Profits: Yes
Alternate Antibody Name: CD49e, CD51
Gene Symbol: ITGAV/ITGB5
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2619586
Uniprot ID: P06756 P18084
Entrez Gene ID: 3685 3693
Clonality: Monoclonal
Immunogen: small cell lung carcinoma from the UCLA P3 cell line expressing the vitronectin receptor.
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: No
Antigen Name: integrin alpha V beta 5 (CD51, fibronectin/vitronectin receptor)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: Apparent: 160 kDa (alpha chain), 95 kDa (beta chain)
Depositor: Wayner, E.A.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: Immunostaining works only on acetone fixed preps. P1F6 completely inhibits alphaVbeta5 dependent binding to vitronectin-coated substrates. Doesn't cross-reactwith alphaVbeta3.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Hamster, Human, Rat
Additional Information: P1F6 recognizes a composite epitope on alphaV/beta5 native heterodimer integrin.
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1F6 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P1F6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface.
Cheresh DA
The Journal of cell biology 113.4 (1991 May): 919-29.