P1F6

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$40.00
SKU: P1F6
View product citations for antibody P1F6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha V beta 5 (CD51, fibronectin/vitronectin receptor)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Chicken, Hamster, Human, Rat
Depositors Notes: Immunostaining works only on acetone fixed preps. P1F6 completely inhibits alphaVbeta5 dependent binding to vitronectin-coated substrates. Doesn't cross-reactwith alphaVbeta3.
Antigen Molecular Weight: Apparent: 160 kDa (alpha chain), 95 kDa (beta chain)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGAV/ITGB5
Immunogen: small cell lung carcinoma from the UCLA P3 cell line expressing the vitronectin receptor.
Alternate Gene Names:
Alternate Antibody Name: CD49e, CD51
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence:
Uniprot ID: P06756 P18084 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3685 3693 
Additional Characterization:  
Antibody Registry ID: AB_2619586 
Additional Information: P1F6 recognizes a composite epitope on alphaV/beta5 native heterodimer integrin.
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1F6 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P1F6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References

    Myofibroblast contraction activates latent TGF-beta1 from the extracellular matrix.
    Hinz B
    The Journal of cell biology 179.6 (2007 Dec 17): 1311-23.

    The alphavbeta5 integrin functions as an endocytic receptor for vitronectin.
    McKeown-Longo P
    Journal of cell science 111 ( Pt 4). (1998 Feb): 425-33.

    IHC References
    IP References
    FB References

    Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface.
    Cheresh DA
    The Journal of cell biology 113.4 (1991 May): 919-29.

    Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor.
    Sheppard D
    The Journal of biological chemistry 269.9 (1994 Mar 4): 6940-8.

    Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells.
    Clegg DO
    Stem cells (Dayton, Ohio) 27.10 (2009 Oct): 2427-34.

    Increased local expression of coagulation factor X contributes to the fibrotic response in human and murine lung injury.
    Chambers RC
    The Journal of clinical investigation 119.9 (2009 Sep): 2550-63.

    Contortrostatin, a homodimeric disintegrin, binds to integrin alphavbeta5.
    Markland FS
    Biochemical and biophysical research communications 267.1 (2000 Jan 7): 350-5.

    Chondrocytes attach to hyaline or calcified cartilage and bone.
    Kandel RA
    Osteoarthritis and cartilage 12.1 (2004 Jan): 56-64.

    Integrin alpha v beta 5-dependent serine phosphorylation of paxillin in cultured human macrophages adherent to vitronectin.
    Yamada KM
    The Journal of biological chemistry 271.18 (1996 May 3): 11016-22.

    FACS References
    All References

    Increased local expression of coagulation factor X contributes to the fibrotic response in human and murine lung injury.
    Chambers RC
    The Journal of clinical investigation 119.9 (2009 Sep): 2550-63.

    Myofibroblast contraction activates latent TGF-beta1 from the extracellular matrix.
    Hinz B
    The Journal of cell biology 179.6 (2007 Dec 17): 1311-23.

    The alphavbeta5 integrin functions as an endocytic receptor for vitronectin.
    McKeown-Longo P
    Journal of cell science 111 ( Pt 4). (1998 Feb): 425-33.

    Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor.
    Sheppard D
    The Journal of biological chemistry 269.9 (1994 Mar 4): 6940-8.

    Alpha(v)beta3 and alpha(v)beta5 integrins bind both the proximal RGD site and non-RGD motifs within noncollagenous (NC1) domain of the alpha3 chain of type IV collagen: implication for the mechanism of endothelia cell adhesion.
    Hudson BG
    The Journal of biological chemistry 279.4 (2004 Jan 23): 2772-80.

    Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface.
    Cheresh DA
    The Journal of cell biology 113.4 (1991 May): 919-29.

    Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells.
    Clegg DO
    Stem cells (Dayton, Ohio) 27.10 (2009 Oct): 2427-34.

    Contortrostatin, a homodimeric disintegrin, binds to integrin alphavbeta5.
    Markland FS
    Biochemical and biophysical research communications 267.1 (2000 Jan 7): 350-5.

    Chondrocytes attach to hyaline or calcified cartilage and bone.
    Kandel RA
    Osteoarthritis and cartilage 12.1 (2004 Jan): 56-64.

    Integrin alpha v beta 5-dependent serine phosphorylation of paxillin in cultured human macrophages adherent to vitronectin.
    Yamada KM
    The Journal of biological chemistry 271.18 (1996 May 3): 11016-22.

    The opsonin MFG-E8 is a ligand for the alphavbeta5 integrin and triggers DOCK180-dependent Rac1 activation for the phagocytosis of apoptotic cells.
    Birge RB
    Experimental cell research 292.2 (2004 Jan 15): 403-16.

    Integrins beta 5, beta 3 and alpha v are apically distributed in endometrial epithelium.
    Vićovac L
    Molecular human reproduction 2.7 (1996 Jul): 527-34.

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