P1F6
Catalog Fields
Antigen: integrin alpha V beta 5 (CD51, fibronectin/vitronectin receptor)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Chicken, Hamster, Human, Rat
Depositors Notes: Immunostaining works only on acetone fixed preps. P1F6 completely inhibits alphaVbeta5 dependent binding to vitronectin-coated substrates. Doesn't cross-reactwith alphaVbeta3.
Predicted Species Reactivity:
Gene: ITGAV/ITGB5
Immunogen: small cell lung carcinoma from the UCLA P3 cell line expressing the vitronectin receptor.
Alternate Gene Names:
Alternate Antibody Name: CD49e, CD51
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence:
Uniprot ID: P06756 P18084
Immunogen Sequence: Full length protein
Entrez Gene ID: 3685 3693
Antibody Registry ID: AB_2619586
Additional Information: P1F6 recognizes a composite epitope on alphaV/beta5 native heterodimer integrin.
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1F6 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P1F6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface.
Cheresh DA
The Journal of cell biology 113.4 (1991 May): 919-29.