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DSHB Data Sheet

Catalog Fields

Product Name/ID: P1H6
Available to For-Profits: Yes
Alternate Antibody Name: CD49b
Gene Name: ITGA2
Ab Isotype: MIgG3
Gene Symbol:
Antibody Registry ID: AB_2619599 
Uniprot ID: P17301 
Entrez Gene ID: 3673 
Clonality: Monoclonal
Immunogen: Human HT-1080 fibrosarcoma cells
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: No
Antigen Name: integrin alpha 2 (CD49b)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: Predicted: 129 kDa; Apparent: 150 kDa
Depositor: Wayner, E.A. / Carter, W.G.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: Immunostaining with frozen or acetone fixation. Doesn't work for FFPE. No function inhibition.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: P1H6 recognizes an epitope that requires activation of the alpha2/beta1 complex.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1H6 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1H6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    FACS References
    All References

    The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells.
    Kaur P
    The Journal of cell biology 110.4 (1990 Apr): 1387-404.

    Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin.
    Wilke MS
    The American journal of pathology 148.5 (1996 May): 1445-61.

    Interaction of integrins alpha 3 beta 1 and alpha 2 beta 1: potential role in keratinocyte intercellular adhesion.
    Carter WG
    The Journal of cell biology 120.2 (1993 Jan): 523-35.

    Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
    Carter WG
    The Journal of cell biology 105.4 (1987 Oct): 1873-84.

    Integrin expression in human neuroblastoma cells with or without N-myc amplification and in ectopic/orthotopic nude mouse tumors.
    Culp LA
    Experimental cell research 213.1 (1994 Jul): 156-63.

    Dynamic expression of alpha 1 beta 1 and alpha 2 beta 1 integrin receptors by human vascular smooth muscle cells. Alpha 2 beta 1 integrin is required for chemotaxis across type I collagen-coated membranes.
    Ross R
    The American journal of pathology 145.5 (1994 Nov): 1070-81.

    Fibrillar collagen inhibits arterial smooth muscle proliferation through regulation of Cdk2 inhibitors.
    Ross R
    Cell 87.6 (1996 Dec 13): 1069-78.

    Degraded collagen fragments promote rapid disassembly of smooth muscle focal adhesions that correlates with cleavage of pp125(FAK), paxillin, and talin.
    Raines EW
    The Journal of cell biology 147.3 (1999 Nov 1): 619-30.

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