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SKU: P2X7-L4

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DSHB Data Sheet

Catalog Fields

Product Name/ID: P2X7-L4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: P2RX7
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_2618074 
Uniprot ID: Q99572 
Entrez Gene ID: 5027 
Clonality: Monoclonal
Immunogen: hP2X7 transfected into mouse XS63 myeloma cell line
Immunogen Sequence: Full length sequence
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Purinergic receptor P2X 7
Epitope Location or Sequence: The epitope contains arginine 307.
Alternate Antigen Name:
Deposit Date: 2/27/2014
Antigen Molecular Weight: 68.5kDa
Depositor: Gu, B.
Antigen Sequence:
Depositor Institution: Florey Institute of Neuroscience & Mental Health, Australia
Antigen Species: Human
Depositor Notes: The Purinergic receptor P2X 7 is an ATP receptor and localized to the Golgi apparatus, predominantly in brain and immune tissues.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Rabbit, Sheep
Additional Information: RRID:AB_2618074
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P2X7-L4 was deposited to the DSHB by Gu, B. (DSHB Hybridoma Product P2X7-L4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • All References
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