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SKU: P2X7-L4
View product citations for antibody P2X7-L4 on CiteAb

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Available: 35

DSHB Data Sheet

Catalog Fields

Antigen: Purinergic receptor P2X 7
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Gu, B.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Florey Institute of Neuroscience & Mental Health, Australia
Positive Tested Species Reactivity: Human, Rabbit, Sheep
Depositors Notes: The Purinergic receptor P2X 7 is an ATP receptor and localized to the Golgi apparatus, predominantly in brain and immune tissues.
Antigen Molecular Weight: 68.5kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: P2RX7
Immunogen: hP2X7 transfected into mouse XS63 myeloma cell line
Alternate Gene Names: MGC20089, P2X7
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: The epitope contains arginine 307.
Uniprot ID: Q99572 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 5027 
Additional Characterization:  
Antibody Registry ID: AB_2618074 
Additional Information: RRID:AB_2618074
Recommended Applications: ELISA, FACS, Function Blocking, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P2X7-L4 was deposited to the DSHB by Gu, B. (DSHB Hybridoma Product P2X7-L4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IHC References
  • IP References
  • ELISA References
  • FB References
  • All References
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