P4G9

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$40.00
SKU: P4G9
View product citations for antibody P4G9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha-4 (CD49d)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center,
Positive Tested Species Reactivity: Human
Depositors Notes: P4G9 stimulates aggregation of T cells. IHC is validated using cryostat frozen sections.
Antigen Molecular Weight: 114.9 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGA4
Immunogen: HT1080 human fibrosarcoma cell line
Alternate Gene Names: CD49D
Alternate Antibody Name: CD49d
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: N-terminus 1-100
Uniprot ID: P13612 
Immunogen Sequence:
Entrez Gene ID: 3676 
Additional Characterization:  
Antibody Registry ID: AB_2619601 
Additional Information: RRID:AB_2619601
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4G9 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IHC References
    IP References
    FB References
    FACS References

    Molecular mapping of functional antibody binding sites of alpha 4 integrin.
    Osborn L
    The Journal of biological chemistry 270.24 (1995 Jun 16): 14270-3.

    Epitope Map References

    Molecular mapping of functional antibody binding sites of alpha 4 integrin.
    Osborn L
    The Journal of biological chemistry 270.24 (1995 Jun 16): 14270-3.

    All References

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