P4G9
Catalog Fields
Antigen: integrin alpha-4 (CD49d)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG2b
Depositors Institution: Fred Hutchison Cancer Research Center,
Host Species: mouse
Depositors Notes: P4G9 stimulates aggregation of T cells. IHC is validated using cryostat frozen sections.
Positive Tested Species Reactivity: Human
Gene: ITGA4
Antigen Molecular Weight: 114.9 kDa
Alternate Gene Names: CD49D
Immunogen: HT1080 human fibrosarcoma cell line
Clonality: Monoclonal
Alternate Antibody Name: CD49d
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: N-terminus 1-100
Uniprot ID: P13612
Additional Information: RRID:AB_2619601
Entrez Gene ID: 3676
Antibody Registry ID: AB_2619601
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4G9 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
5 References
Initial Publication
Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
Carter WG
The Journal of cell biology 109.3 (1989 Sep): 1321-30.