P4G9

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$45.00 to $425.00
SKU: P4G9
View product citations for antibody P4G9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Product Name/ID: P4G9
Available to For-Profits: Yes
Alternate Antibody Name: CD49d
Gene Name: ITGA4
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_2619601 
Uniprot ID: P13612 
RRID:  
Entrez Gene ID: 3676 
Clonality: Monoclonal
Immunogen: HT1080 human fibrosarcoma cell line
Clone:
Immunogen Sequence:
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Antigen Name: integrin alpha-4 (CD49d)
Epitope Location or Sequence: N-terminus 1-100
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: 114.9 kDa
Depositor: Wayner, E.A. / Carter, W.G.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: P4G9 stimulates aggregation of T cells. IHC is validated using cryostat frozen sections.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: RRID:AB_2619601
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4G9 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P4G9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IHC References
    IP References
    FB References
    FACS References

    Molecular mapping of functional antibody binding sites of alpha 4 integrin.
    Osborn L
    The Journal of biological chemistry 270.24 (1995 Jun 16): 14270-3.

    Epitope Map References

    Molecular mapping of functional antibody binding sites of alpha 4 integrin.
    Osborn L
    The Journal of biological chemistry 270.24 (1995 Jun 16): 14270-3.

    All References

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