P5D8
Catalog Fields
Antigen: integrin beta-1 (CD29), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A.
Isotype: MIgM
Depositors Institution: Fred Hutchison Cancer Research Center
Host Species: mouse
Depositors Notes: Inhibits cell adhesion to laminin, fibronectin and collagen I or III, and activates lymphocyte adhesion to laminin, fibronectin and collagen I or III. This antibody doesn't appear to work for western blotting.
Positive Tested Species Reactivity: Human
Gene: ITGB1
Antigen Molecular Weight: Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa
Alternate Gene Names: CD29, GPIIA, FNRB, MDF2, MSK12, VLA-4beta
Immunogen: Human fibrosarcoma HT-1080 cells
Clonality: Monoclonal
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Uniprot ID: P05556
Epitope Location or Sequence: extracellular domain
Entrez Gene ID: 3688
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2619592
Recommended Applications: Function Blocking, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5D8 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P5D8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
Carter WG
The Journal of cell biology 105.4 (1987 Oct): 1873-84.