Catalog Fields
Antigen: integrin, alpha-V beta-5 heterodimer
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Human
Depositors Notes:
Predicted Species Reactivity:
Gene: ITGAV/ITGB5
Immunogen: HT1080 human fibrosarcoma cell line
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence:
Uniprot ID: P06756
Immunogen Sequence:
Entrez Gene ID: 3685/3693
Antibody Registry ID: AB_2619594
Additional Information: RRID:AB_2619594
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5H9 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P5H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
Carter WG
The Journal of cell biology 109.3 (1989 Sep): 1321-30.