P5H9
Catalog Fields
Antigen: integrin, alpha-V beta-5 heterodimer
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center
Positive Tested Species Reactivity: Human
Depositors Notes:
Antigen Molecular Weight: 116.0 & 88.0 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: ITGAV/ITGB5
Immunogen: HT1080 human fibrosarcoma cell line
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence:
Uniprot ID: P06756
Immunogen Sequence:
Entrez Gene ID: 3685/3693
Additional Characterization:
Antibody Registry ID: AB_2619594
Additional Information: RRID:AB_2619594
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P5H9 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P5H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
4 References
Initial Publication
Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin.
Carter WG
The Journal of cell biology 109.3 (1989 Sep): 1321-30.