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SKU: P8D4-s
View product citations for antibody P8D4 on CiteAb

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Available: 14

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha5beta1, Xenopus
Available to For-Profits: Yes
Antigen Species: Xenopus laevis
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1
Depositor: DeSimone, D.W.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Xenopus laevis
Depositors Institution: University of Virginia School of Medicine
Antigen Molecular Weight: 135 kDa reduced, 155kDa unreduced
Depositors Notes: This antibody blocks binding of alpha5 beta 1 integrin to the synergy site in type III-9 fibrobnectin repeat.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: S3-1 cells (derived from St. 18 dorsal explant)
Gene: itga5
Alternate Antibody Name:
Alternate Gene Names: VLA-5
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SpZ/O-Ag 14
Epitope Mapped: No
Uniprot ID: Q06274 
Epitope Location or Sequence: recognizes a composite epitope on alpha5/beta1 native heterodimer integrin.
Entrez Gene ID: 394366 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2722121 
Additional Characterization:  
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation
Additional Information: RRID:AB_2722121
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P8D4 was deposited to the DSHB by DeSimone, D.W. (DSHB Hybridoma Product P8D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

6 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References

    Integrin alpha5beta1 supports the migration of Xenopus cranial neural crest on fibronectin.
    DeSimone DW
    Developmental biology 260.2 (2003 Aug 15): 449-64.

    IHC References
    IP References
    FB References
    All References

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