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SKU: P8D4-s
View product citations for antibody P8D4 on CiteAb

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Available: 33

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha5beta1, Xenopus
Hybridoma Cells Available:
Antigen Species: Xenopus laevis
Depositor: DeSimone, D.W.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia School of Medicine
Positive Tested Species Reactivity: Xenopus laevis
Depositors Notes: This antibody blocks binding of alpha5 beta 1 integrin to the synergy site in type III-9 fibrobnectin repeat.
Antigen Molecular Weight: 135 kDa reduced, 155kDa unreduced
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: itga5
Immunogen: S3-1 cells (derived from St. 18 dorsal explant)
Alternate Gene Names: VLA-5
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SpZ/O-Ag 14
Epitope Location or Sequence: recognizes a composite epitope on alpha5/beta1 native heterodimer integrin.
Uniprot ID: Q06274 
Immunogen Sequence: Full length protein
Entrez Gene ID: 394366 
Additional Characterization:
Antibody Registry ID: AB_2722121 
Additional Information: RRID:AB_2722121
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P8D4 was deposited to the DSHB by DeSimone, D.W. (DSHB Hybridoma Product P8D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References

    Integrin alpha5beta1 supports the migration of Xenopus cranial neural crest on fibronectin.
    DeSimone DW
    Developmental biology 260.2 (2003 Aug 15): 449-64.

    IHC References
    IP References
    FB References
    All References

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