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SKU: P8D4-s
View product citations for antibody P8D4 on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha5beta1, Xenopus
Hybridoma Cells Available: No
Antigen Species: Xenopus laevis
Depositor: DeSimone, D.W.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia School of Medicine
Positive Tested Species Reactivity: Xenopus laevis
Depositors Notes: This antibody blocks binding of alpha5 beta 1 integrin to the synergy site in type III-9 fibrobnectin repeat.
Antigen Molecular Weight: 135 kDa reduced, 155kDa unreduced
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: itga5
Immunogen: S3-1 cells (derived from St. 18 dorsal explant)
Alternate Gene Names: VLA-5
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SpZ/O-Ag 14
Epitope Location or Sequence: recognizes a composite epitope on alpha5/beta1 native heterodimer integrin.
Uniprot ID: Q06274 
Immunogen Sequence: Full length protein
Entrez Gene ID: 394366 
Additional Characterization:  
Antibody Registry ID: AB_2722121 
Additional Information: RRID:AB_2722121
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P8D4 was deposited to the DSHB by DeSimone, D.W. (DSHB Hybridoma Product P8D4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References

    Integrin alpha5beta1 supports the migration of Xenopus cranial neural crest on fibronectin.
    DeSimone DW
    Developmental biology 260.2 (2003 Aug 15): 449-64.

    IHC References
    IP References
    FB References
    All References

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