PAM 18E5

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$40.00
SKU: PAM 18E5
View product citations for antibody PAM 18E5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: PAM; peptidyl-glycine alpha-amidating monooxygenase; enzymatic luminal domain
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Mains, R.E.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Connecticut Health Center
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: The 18E5 antibody recognizes the monooxygenase enzymatic luminal domain of PAM. This antibody is also known as PAM-PHM.
Antigen Molecular Weight: 80 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Pam
Immunogen: Recombinant dual enzymatic domains of rPAM-1 expressed in HEK-293 and purified
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3.653
Epitope Location or Sequence: PHM (monooxxygenase domain)
Uniprot ID: P14925 
Immunogen Sequence: a.a. 36-866
Entrez Gene ID: 25508 
Additional Characterization:
Antibody Registry ID: AB_2722122 
Additional Information: RRID:AB_2722122
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PAM 18E5 was deposited to the DSHB by Mains, R.E. (DSHB Hybridoma Product PAM 18E5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    Epitope Map References
    All References

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