PAM 18E5

(0)No Reviews yet
$50.00 to $600.00

In Stock

Available: 40

DSHB Data Sheet

Catalog Fields

Product Name/ID: PAM 18E5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Pam
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_2722122 
Uniprot ID: P14925 
Entrez Gene ID: 25508 
Clonality: Monoclonal
Immunogen: Recombinant dual enzymatic domains of rPAM-1 expressed in HEK-293 and purified
Immunogen Sequence: a.a. 36-866
Myeloma Strain: P3.653
Epitope Mapped: Yes
Antigen Name: PAM; peptidyl-glycine alpha-amidating monooxygenase; enzymatic luminal domain
Epitope Location or Sequence: PHM (monooxxygenase domain)
Alternate Antigen Name:
Deposit Date: 8/15/2017
Antigen Molecular Weight: 80 kDa
Depositor: Mains, R.E.
Antigen Sequence:
Depositor Institution: University of Connecticut Health Center
Antigen Species: Rat
Depositor Notes: The 18E5 antibody recognizes the monooxygenase enzymatic luminal domain of PAM. This antibody is also known as PAM-PHM.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse, Rat
Additional Information: RRID:AB_2722122
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PAM 18E5 was deposited to the DSHB by Mains, R.E. (DSHB Hybridoma Product PAM 18E5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    Epitope Map References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review